Characterisation of Waterborne Psychrophilic Massilia Isolates
with Violacein Production and Description of Massilia
antarctica sp. nov.

J 2022

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Hans-Jürgen BUSSE, Vendula KOUBLOVÁ, Stanislava KRÁLOVÁ et. al.

Basic information

Original name

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

Authors

SEDLÁČEK, Ivo (203 Czech Republic, guarantor, belonging to the institution), Pavla HOLOCHOVÁ (203 Czech Republic, belonging to the institution), Hans-Jürgen BUSSE, Vendula KOUBLOVÁ (203 Czech Republic, belonging to the institution), Stanislava KRÁLOVÁ (703 Slovakia, belonging to the institution), Pavel ŠVEC (203 Czech Republic, belonging to the institution), Roman SOBOTKA, Eva STAŇKOVÁ (203 Czech Republic, belonging to the institution), Jan PILNÝ, Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Jana SMOLÍKOVÁ and Karel SEDLÁŘ

Edition

Microorganisms, MDPI, 2022, 2076-2607

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.500

RIV identification code

RIV/00216224:14310/22:00125595

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/microorganisms10040704

UT WoS

000786017300001

Keywords in English

Massilia; violacein; psychrophilic; description; whole-genome sequencing; Antarctica

Abstract

V originále

A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398T were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6–100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA–DNA hybridisation values clearly proved that P8398T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398T (= CCM 8941T = LMG 32108T).

Links

EF18_046/0015974, research and development project

Name: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2015078, research and development project

Name: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)

Investor: Ministry of Education, Youth and Sports of the CR

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Hans-Jürgen BUSSE, Vendula KOUBLOVÁ, Stanislava KRÁLOVÁ, Pavel ŠVEC, Roman SOBOTKA, Eva STAŇKOVÁ, Jan PILNÝ, Ondrej ŠEDO, Jana SMOLÍKOVÁ and Karel SEDLÁŘ. Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. Microorganisms. MDPI, 2022, vol. 10, No 4, p. 1-14. ISSN 2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms10040704.

@article{1843919,
   author = {Sedláček, Ivo and Holochová, Pavla and Busse, HansandJürgen and Koublová, Vendula and Králová, Stanislava and Švec, Pavel and Sobotka, Roman and Staňková, Eva and Pilný, Jan and Šedo, Ondrej and Smolíková, Jana and Sedlář, Karel},
   article_number = {4},
   doi = {http://dx.doi.org/10.3390/microorganisms10040704},
   keywords = {Massilia; violacein; psychrophilic; description; whole-genome sequencing; Antarctica},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.},
   url = {https://www.mdpi.com/2076-2607/10/4/704},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 1843919
AU  - Sedláček, Ivo - Holochová, Pavla - Busse, Hans-Jürgen - Koublová, Vendula - Králová, Stanislava - Švec, Pavel - Sobotka, Roman - Staňková, Eva - Pilný, Jan - Šedo, Ondrej - Smolíková, Jana - Sedlář, Karel
PY  - 2022
TI  - Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.
JF  - Microorganisms
VL  - 10
IS  - 4
SP  - 1-14
EP  - 1-14
PB  - MDPI
SN  - 20762607
KW  - Massilia
KW  - violacein
KW  - psychrophilic
KW  - description
KW  - whole-genome sequencing
KW  - Antarctica
UR  - https://www.mdpi.com/2076-2607/10/4/704
N2  - A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398T were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6–100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA–DNA hybridisation values clearly proved that P8398T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398T (= CCM 8941T = LMG 32108T).
ER  -

SEDLÁČEK, Ivo, Pavla HOLOCHOVÁ, Hans-Jürgen BUSSE, Vendula KOUBLOVÁ, Stanislava KRÁLOVÁ, Pavel ŠVEC, Roman SOBOTKA, Eva STAŇKOVÁ, Jan PILNÝ, Ondrej ŠEDO, Jana SMOLÍKOVÁ and Karel SEDLÁŘ. Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. \textit{Microorganisms}. MDPI, 2022, vol.~10, No~4, p.~1-14. ISSN~2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms10040704.

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