PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin

SHAPOVAL, Oleksandr, Julian BRANDMEIER, Mykhailo NAHORNIAK, Viktoriia OLEKSA, Ekaterina MAKHNEVA, Hans-Heiner GORRIS, Zdeněk FARKA and Daniel HORÁK. PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin. Talanta. Elsevier, 2022, vol. 244, No 123400, p. 1-8. ISSN 0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2022.123400.

Basic information

• Original name: PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin
• Authors: SHAPOVAL, Oleksandr (804 Ukraine), Julian BRANDMEIER (276 Germany, belonging to the institution), Mykhailo NAHORNIAK (804 Ukraine), Viktoriia OLEKSA (804 Ukraine), Ekaterina MAKHNEVA (643 Russian Federation, belonging to the institution), Hans-Heiner GORRIS (276 Germany, belonging to the institution), Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution) and Daniel HORÁK.
• Edition: Talanta, Elsevier, 2022, 0039-9140.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 6.100
• RIV identification code: RIV/00216224:14310/22:00125622
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1016/j.talanta.2022.123400
• UT WoS: 000793049100006
• Keywords in English: Photon-upconversion nanoparticle; Poly(methyl vinyl ether‐alt‐maleic acid);Bioconjugation; Immunoassay; Upconversion-linked immunosorbent assay; Cardiac troponin I
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Surface engineering of upconverting nanoparticles (UCNPs) is crucial for their bioanalytical applications. Here, an antibody specific to cardiac troponin I (cTnI), an important biomarker for acute myocardial infection, was covalently immobilized on the surface of UCNPs to prepare a label for the detection of cTnI biomarker in an upconversion-linked immunoassay (ULISA). Core-shell UCNPs (NaYF4:Yb,Tm@NaYF4) were first coated with poly(methyl vinyl ether‐alt‐maleic acid) (PMVEMA) and then conjugated to antibodies. The morphology (size and uniformity), hydrodynamic diameter, chemical composition, and amount of coating on the of UCNPs, as well as their upconversion luminescence, colloidal stability, and leaching of Y3+ ions into the surrounding media, were determined. The developed ULISA allowed reaching a limit of detection (LOD) of 0.13 ng/ml and 0.25 ng/ml of cTnI in plasma and serum, respectively, which represents 12- and 2-fold improvement to conventional enzyme-linked immunosorbent based on the same immunoreagents.

Links

• GA21-03156S, research and development project: Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

• LTAB19011, research and development project: Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION