PMVEMA-coated upconverting nanoparticles for
upconversion-linked immunoassay of cardiac troponin

J 2022

PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin

SHAPOVAL, Oleksandr, Julian BRANDMEIER, Mykhailo NAHORNIAK, Viktoriia OLEKSA, Ekaterina MAKHNEVA et. al.

Basic information

Original name

PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin

Authors

SHAPOVAL, Oleksandr (804 Ukraine), Julian BRANDMEIER (276 Germany, belonging to the institution), Mykhailo NAHORNIAK (804 Ukraine), Viktoriia OLEKSA (804 Ukraine), Ekaterina MAKHNEVA (643 Russian Federation, belonging to the institution), Hans-Heiner GORRIS (276 Germany, belonging to the institution), Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution) and Daniel HORÁK

Edition

Talanta, Elsevier, 2022, 0039-9140

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.100

RIV identification code

RIV/00216224:14310/22:00125622

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.talanta.2022.123400

UT WoS

000793049100006

Keywords in English

Photon-upconversion nanoparticle; Poly(methyl vinyl ether‐alt‐maleic acid);Bioconjugation; Immunoassay; Upconversion-linked immunosorbent assay; Cardiac troponin I

Abstract

V originále

Surface engineering of upconverting nanoparticles (UCNPs) is crucial for their bioanalytical applications. Here, an antibody specific to cardiac troponin I (cTnI), an important biomarker for acute myocardial infection, was covalently immobilized on the surface of UCNPs to prepare a label for the detection of cTnI biomarker in an upconversion-linked immunoassay (ULISA). Core-shell UCNPs (NaYF4:Yb,Tm@NaYF4) were first coated with poly(methyl vinyl ether‐alt‐maleic acid) (PMVEMA) and then conjugated to antibodies. The morphology (size and uniformity), hydrodynamic diameter, chemical composition, and amount of coating on the of UCNPs, as well as their upconversion luminescence, colloidal stability, and leaching of Y3+ ions into the surrounding media, were determined. The developed ULISA allowed reaching a limit of detection (LOD) of 0.13 ng/ml and 0.25 ng/ml of cTnI in plasma and serum, respectively, which represents 12- and 2-fold improvement to conventional enzyme-linked immunosorbent based on the same immunoreagents.

Links

GA21-03156S, research and development project

Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

LTAB19011, research and development project

Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION

Citovat

SHAPOVAL, Oleksandr, Julian BRANDMEIER, Mykhailo NAHORNIAK, Viktoriia OLEKSA, Ekaterina MAKHNEVA, Hans-Heiner GORRIS, Zdeněk FARKA and Daniel HORÁK. PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin. Talanta. Elsevier, 2022, vol. 244, No 123400, p. 1-8. ISSN 0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2022.123400.

@article{1845339,
   author = {Shapoval, Oleksandr and Brandmeier, Julian and Nahorniak, Mykhailo and Oleksa, Viktoriia and Makhneva, Ekaterina and Gorris, HansandHeiner and Farka, Zdeněk and Horák, Daniel},
   article_number = {123400},
   doi = {http://dx.doi.org/10.1016/j.talanta.2022.123400},
   keywords = {Photon-upconversion nanoparticle; Poly(methyl vinyl ether‐alt‐maleic acid);Bioconjugation; Immunoassay; Upconversion-linked immunosorbent assay; Cardiac troponin I},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin},
   url = {https://www.sciencedirect.com/science/article/pii/S0039914022001965},
   volume = {244},
   year = {2022}
}

TY  - JOUR
ID  - 1845339
AU  - Shapoval, Oleksandr - Brandmeier, Julian - Nahorniak, Mykhailo - Oleksa, Viktoriia - Makhneva, Ekaterina - Gorris, Hans-Heiner - Farka, Zdeněk - Horák, Daniel
PY  - 2022
TI  - PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin
JF  - Talanta
VL  - 244
IS  - 123400
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 00399140
KW  - Photon-upconversion nanoparticle
KW  - Poly(methyl vinyl ether‐alt‐maleic acid);Bioconjugation
KW  - Immunoassay
KW  - Upconversion-linked immunosorbent assay
KW  - Cardiac troponin I
UR  - https://www.sciencedirect.com/science/article/pii/S0039914022001965
N2  - Surface engineering of upconverting nanoparticles (UCNPs) is crucial for their bioanalytical applications. Here, an antibody specific to cardiac troponin I (cTnI), an important biomarker for acute myocardial infection, was covalently immobilized on the surface of UCNPs to prepare a label for the detection of cTnI biomarker in an upconversion-linked immunoassay (ULISA). Core-shell UCNPs (NaYF4:Yb,Tm@NaYF4) were first coated with poly(methyl vinyl ether‐alt‐maleic acid) (PMVEMA) and then conjugated to antibodies. The morphology (size and uniformity), hydrodynamic diameter, chemical composition, and amount of coating on the of UCNPs, as well as their upconversion luminescence, colloidal stability, and leaching of Y3+ ions into the surrounding media, were determined. The developed ULISA allowed reaching a limit of detection (LOD) of 0.13 ng/ml and 0.25 ng/ml of cTnI in plasma and serum, respectively, which represents 12- and 2-fold improvement to conventional enzyme-linked immunosorbent based on the same immunoreagents.
ER  -

SHAPOVAL, Oleksandr, Julian BRANDMEIER, Mykhailo NAHORNIAK, Viktoriia OLEKSA, Ekaterina MAKHNEVA, Hans-Heiner GORRIS, Zdeněk FARKA and Daniel HORÁK. PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin. \textit{Talanta}. Elsevier, 2022, vol.~244, No~123400, p.~1-8. ISSN~0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2022.123400.

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