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@article{1846417,
author = {Vrchovecká, Kateřina and Pávková Goldbergová, Monika and Engqvist, H. and PujariandPalmer, M.},
article_location = {Basel},
article_number = {4},
doi = {http://dx.doi.org/10.3390/biomedicines10040736},
keywords = {phosphoserine; bone tissue adhesive; calcium phosphate cement; odontoblast; osteoblast; cytotoxicity; ion release; in vitro},
language = {eng},
issn = {2227-9059},
journal = {Biomedicines},
title = {Cytocompatibility and Bioactive Ion Release Profiles of Phosphoserine Bone Adhesive: Bridge from In Vitro to In Vivo},
url = {https://www.mdpi.com/2227-9059/10/4/736#cite},
volume = {10},
year = {2022}
}
TY - JOUR
ID - 1846417
AU - Vrchovecká, Kateřina - Pávková Goldbergová, Monika - Engqvist, H. - Pujari-Palmer, M.
PY - 2022
TI - Cytocompatibility and Bioactive Ion Release Profiles of Phosphoserine Bone Adhesive: Bridge from In Vitro to In Vivo
JF - Biomedicines
VL - 10
IS - 4
SP - 1-29
EP - 1-29
PB - MDPI
SN - 22279059
KW - phosphoserine
KW - bone tissue adhesive
KW - calcium phosphate cement
KW - odontoblast
KW - osteoblast
KW - cytotoxicity
KW - ion release
KW - in vitro
UR - https://www.mdpi.com/2227-9059/10/4/736#cite
N2 - One major challenge when developing new biomaterials is translating in vitro testing to in vivo models. We have recently shown that a single formulation of a bone tissue adhesive, phosphoserine modified cement (PMC), is safe and resorbable in vivo. Herein, we screened many new adhesive formulations, for cytocompatibility and bioactive ion release, with three cell lines: MDPC23 odontoblasts, MC3T3 preosteoblasts, and L929 fibroblasts. Most formulations were cytocompatible by indirect contact testing (ISO 10993-12). Formulations with larger amounts of phosphoserine (>50%) had delayed setting times, greater ion release, and cytotoxicity in vitro. The trends in ion release from the adhesive that were cured for 24 h (standard for in vitro) were similar to release from the adhesives cured only for 5–10 min (standard for in vivo), suggesting that we may be able to predict the material behavior in vivo, using in vitro methods. Adhesives containing calcium phosphate and silicate were both cytocompatible for seven days in direct contact with cell monolayers, and ion release increased the alkaline phosphatase (ALP) activity in odontoblasts, but not pre-osteoblasts. This is the first study evaluating how PMC formulation affects osteogenic cell differentiation (ALP), cytocompatibility, and ion release, using in situ curing conditions similar to conditions in vivo.
ER -
VRCHOVECKÁ, Kateřina, Monika PÁVKOVÁ GOLDBERGOVÁ, H. ENGQVIST and M. PUJARI-PALMER. Cytocompatibility and Bioactive Ion Release Profiles of Phosphoserine Bone Adhesive: Bridge from In Vitro to In Vivo. \textit{Biomedicines}. Basel: MDPI, 2022, vol.~10, No~4, p.~1-29. ISSN~2227-9059. Available from: https://dx.doi.org/10.3390/biomedicines10040736.
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