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@article{1846461,
author = {Kotik, M. and Brodsky, K. and Halada, P. and Javurkova, H. and Pelantova, H. and Konvalinkova, D. and Bojarova, P. and Kren, V.},
article_number = {JAN},
doi = {http://dx.doi.org/10.1016/j.catcom.2020.106193},
keywords = {TransglycosylationRutinosyl azideGlycosidaseActivity rescueRutinGlycoside hydrolase family 5},
language = {eng},
issn = {1566-7367},
journal = {CATALYSIS COMMUNICATIONS},
title = {Access to both anomers of rutinosyl azide using wild-type rutinosidase and its catalytic nucleophile mutant},
url = {https://www.sciencedirect.com/science/article/pii/S1566736720302697?via%3Dihub},
volume = {149},
year = {2021}
}
TY - JOUR
ID - 1846461
AU - Kotik, M. - Brodsky, K. - Halada, P. - Javurkova, H. - Pelantova, H. - Konvalinkova, D. - Bojarova, P. - Kren, V.
PY - 2021
TI - Access to both anomers of rutinosyl azide using wild-type rutinosidase and its catalytic nucleophile mutant
JF - CATALYSIS COMMUNICATIONS
VL - 149
IS - JAN
SP - 106193
EP - 106193
SN - 15667367
KW - TransglycosylationRutinosyl azideGlycosidaseActivity rescueRutinGlycoside hydrolase family 5
UR - https://www.sciencedirect.com/science/article/pii/S1566736720302697?via%3Dihub
N2 - Rutinosidases hydrolyze beta-rutinosylated flavonoids. As retaining glycosidases they also have a transglycosylation activity. Here we show that two newly identified wild-type rutinosidases, which are members of the glycoside hydrolase family 5-23, are capable of glycosylation of an inorganic azide with rutin as a glycosyl donor, yielding rutinosyl alpha-azide. On the other hand, rutinosyl beta-azide was synthesized by the catalytic nucleophile mutant of the rutinosidase from Aspergillus niger, which also belongs to GH5-23. Thus, we were able to synthesize at a preparatory scale both anomers of rutinosyl azide from rutin using either wild-type or mutant rutinosidases of GH5-23.
ER -
KOTIK, M., K. BRODSKY, P. HALADA, H. JAVURKOVA, H. PELANTOVA, D. KONVALINKOVA, P. BOJAROVA a V. KREN. Access to both anomers of rutinosyl azide using wild-type rutinosidase and its catalytic nucleophile mutant. Online. \textit{CATALYSIS COMMUNICATIONS}. 2021, roč.~149, JAN, s.~106193-106197. ISSN~1566-7367. Dostupné z: https://dx.doi.org/10.1016/j.catcom.2020.106193. [citováno 2024-04-24]
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