Understanding the toxicity mechanism of CuO nanoparticles: the
intracellular view of exposed earthworm cells

J 2021

Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells

PACHECO, N.N., R. ROUBALOVA, J. DVORAK, O. BENADA, D. PINKAS et. al.

Basic information

Original name

Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells

Authors

PACHECO, N.N., R. ROUBALOVA, J. DVORAK, O. BENADA, D. PINKAS, O. KOFRONOVA, J. SEMERAD, M. PIVOKONSKY, T. CAJTHAML, M. BILEJ and P. PROCHAZKOVA

Edition

ENVIRONMENTAL SCIENCE-NANO, 2021, 2051-8153

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10400 1.4 Chemical sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.473

RIV identification code

RIV/00216224:14740/21:00124533

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1039/d1en00080b

UT WoS

000674973000001

Keywords in English

IN-VITROEISENIA-HORTENSISCOMET ASSAYSOILCELOMOCYTESOXIDEBIOACCUMULATIONBIOAVAILABILITYTERRESTRIALACTIVATION

Abstract

V originále

Copper oxide nanoparticles (CuO NPs) are widely used in industry. Once released, they can enter the soil system and endanger organisms living in this environment. Therefore, monitoring the NP impact on soil organisms and identification of suitable biomarkers associated with NP pollution are required. In this study, immune effector cells of the earthworm Eisenia andrei, amoebocytes, were exposed to environmentally relevant sublethal concentrations of CuO NPs (1, 10, and 100 mu g mL(-1) of Cu) and their impact on the cellular and subcellular levels, as well as on the mRNA levels of molecules involved in the defense reactions, was assessed in vitro. CuO NPs decreased the viability of both amoebocyte subpopulations by 40% at the highest concentration tested (100 mu g mL(-1) of Cu). Further, CuO NPs caused significant attenuation of the phagocytic function of hyaline amoebocytes after 6 and 24 hours of exposure, by 37 and 25%, respectively. The concentration of the lipid peroxidation subproduct, malondialdehyde, was 10 times elevated in cells exposed to CuO NPs (100 mu g mL(-1) of Cu) after 6 hours of exposure. We hypothesize that malondialdehyde may induce DNA breaks, cell cycle arrest, and subsequent cell death. Electron microscopy showed the interaction between CuO NPs and immune effector cells, amoebocytes. Moreover, aggregates of CuO NPs were shown to be engulfed and located in the cytoplasm of these cells. However, data from all experiments indicate that the observed effects of CuO NPs on earthworm coelomocytes were caused mainly by the dissolved Cu2+ ions derived from nanoparticles (NPs). The determination of effective parameters such as oxidative stress, immune reactivity, and genotoxicity would provide valuable comprehension and data for environmental assessment of NP impact on soil organisms.

Links

90043, large research infrastructures

Name: CIISB

Citovat

PACHECO, N.N., R. ROUBALOVA, J. DVORAK, O. BENADA, D. PINKAS, O. KOFRONOVA, J. SEMERAD, M. PIVOKONSKY, T. CAJTHAML, M. BILEJ and P. PROCHAZKOVA. Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells. ENVIRONMENTAL SCIENCE-NANO. 2021, vol. 8, No 9, p. 2464-2477. ISSN 2051-8153. Available from: https://dx.doi.org/10.1039/d1en00080b.

@article{1846843,
   author = {Pacheco, N.N. and Roubalova, R. and Dvorak, J. and Benada, O. and Pinkas, D. and Kofronova, O. and Semerad, J. and Pivokonsky, M. and Cajthaml, T. and Bilej, M. and Prochazkova, P.},
   article_number = {9},
   doi = {http://dx.doi.org/10.1039/d1en00080b},
   keywords = {IN-VITROEISENIA-HORTENSISCOMET ASSAYSOILCELOMOCYTESOXIDEBIOACCUMULATIONBIOAVAILABILITYTERRESTRIALACTIVATION},
   language = {eng},
   issn = {2051-8153},
   journal = {ENVIRONMENTAL SCIENCE-NANO},
   title = {Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells},
   url = {https://pubs.rsc.org/en/content/articlelanding/2021/EN/D1EN00080B},
   volume = {8},
   year = {2021}
}

TY  - JOUR
ID  - 1846843
AU  - Pacheco, N.N. - Roubalova, R. - Dvorak, J. - Benada, O. - Pinkas, D. - Kofronova, O. - Semerad, J. - Pivokonsky, M. - Cajthaml, T. - Bilej, M. - Prochazkova, P.
PY  - 2021
TI  - Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells
JF  - ENVIRONMENTAL SCIENCE-NANO
VL  - 8
IS  - 9
SP  - 2464-2477
EP  - 2464-2477
SN  - 20518153
KW  - IN-VITROEISENIA-HORTENSISCOMET ASSAYSOILCELOMOCYTESOXIDEBIOACCUMULATIONBIOAVAILABILITYTERRESTRIALACTIVATION
UR  - https://pubs.rsc.org/en/content/articlelanding/2021/EN/D1EN00080B
N2  - Copper oxide nanoparticles (CuO NPs) are widely used in industry. Once released, they can enter the soil system and endanger organisms living in this environment. Therefore, monitoring the NP impact on soil organisms and identification of suitable biomarkers associated with NP pollution are required. In this study, immune effector cells of the earthworm Eisenia andrei, amoebocytes, were exposed to environmentally relevant sublethal concentrations of CuO NPs (1, 10, and 100 mu g mL(-1) of Cu) and their impact on the cellular and subcellular levels, as well as on the mRNA levels of molecules involved in the defense reactions, was assessed in vitro. CuO NPs decreased the viability of both amoebocyte subpopulations by 40% at the highest concentration tested (100 mu g mL(-1) of Cu). Further, CuO NPs caused significant attenuation of the phagocytic function of hyaline amoebocytes after 6 and 24 hours of exposure, by 37 and 25%, respectively. The concentration of the lipid peroxidation subproduct, malondialdehyde, was 10 times elevated in cells exposed to CuO NPs (100 mu g mL(-1) of Cu) after 6 hours of exposure. We hypothesize that malondialdehyde may induce DNA breaks, cell cycle arrest, and subsequent cell death. Electron microscopy showed the interaction between CuO NPs and immune effector cells, amoebocytes. Moreover, aggregates of CuO NPs were shown to be engulfed and located in the cytoplasm of these cells. However, data from all experiments indicate that the observed effects of CuO NPs on earthworm coelomocytes were caused mainly by the dissolved Cu2+ ions derived from nanoparticles (NPs). The determination of effective parameters such as oxidative stress, immune reactivity, and genotoxicity would provide valuable comprehension and data for environmental assessment of NP impact on soil organisms.
ER  -

PACHECO, N.N., R. ROUBALOVA, J. DVORAK, O. BENADA, D. PINKAS, O. KOFRONOVA, J. SEMERAD, M. PIVOKONSKY, T. CAJTHAML, M. BILEJ and P. PROCHAZKOVA. Understanding the toxicity mechanism of CuO nanoparticles: the intracellular view of exposed earthworm cells. \textit{ENVIRONMENTAL SCIENCE-NANO}. 2021, vol.~8, No~9, p.~2464-2477. ISSN~2051-8153. Available from: https://dx.doi.org/10.1039/d1en00080b.

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