Other formats:
BibTeX
LaTeX
RIS
@article{1849604,
author = {Rebrošová, Katarína and Samek, Ota and Kizovsky, Martin and Bernatova, Silvie and Holá, Veronika and Růžička, Filip},
article_location = {Lausanne},
article_number = {April 2022},
doi = {http://dx.doi.org/10.3389/fcimb.2022.866463},
keywords = {Raman spectroscopy; Raman tweezers; identification of microorganisms; antimicrobial resistance; microfluidic devices; magnetic beads; diagnostics},
language = {eng},
issn = {2235-2988},
journal = {Frontiers in Cellular and Infection Microbiology},
title = {Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings},
url = {https://www.frontiersin.org/articles/10.3389/fcimb.2022.866463/full},
volume = {12},
year = {2022}
}
TY - JOUR
ID - 1849604
AU - Rebrošová, Katarína - Samek, Ota - Kizovsky, Martin - Bernatova, Silvie - Holá, Veronika - Růžička, Filip
PY - 2022
TI - Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings
JF - Frontiers in Cellular and Infection Microbiology
VL - 12
IS - April 2022
SP - 1-10
EP - 1-10
PB - Frontiers
SN - 22352988
KW - Raman spectroscopy
KW - Raman tweezers
KW - identification of microorganisms
KW - antimicrobial resistance
KW - microfluidic devices
KW - magnetic beads
KW - diagnostics
UR - https://www.frontiersin.org/articles/10.3389/fcimb.2022.866463/full
N2 - Rapid and accurate identification of pathogens causing infections is one of the biggest challenges in medicine. Timely identification of causative agents and their antimicrobial resistance profile can significantly improve the management of infection, lower costs for healthcare, mitigate ever-growing antimicrobial resistance and in many cases, save lives. Raman spectroscopy was shown to be a useful—quick, non-invasive, and non-destructive —tool for identifying microbes from solid and liquid media. Modifications of Raman spectroscopy and/or pretreatment of samples allow single-cell analyses and identification of microbes from various samples. It was shown that those non-culture-based approaches could also detect antimicrobial resistance. Moreover, recent studies suggest that a combination of Raman spectroscopy with optical tweezers has the potential to identify microbes directly from human body fluids. This review aims to summarize recent advances in non-culture-based approaches of identification of microbes and their virulence factors, including antimicrobial resistance, using methods based on Raman spectroscopy in the context of possible use in the future point-of-care diagnostic process.
ER -
REBROŠOVÁ, Katarína, Ota SAMEK, Martin KIZOVSKY, Silvie BERNATOVA, Veronika HOLÁ and Filip RŮŽIČKA. Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings. \textit{Frontiers in Cellular and Infection Microbiology}. Lausanne: Frontiers, 2022, vol.~12, April 2022, p.~1-10. ISSN~2235-2988. Available from: https://dx.doi.org/10.3389/fcimb.2022.866463.
|
