VÁCLAVEK, Tomáš a František FORET. Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds. Electrophoresis. Verlag Chemie, 2023, roč. 44, 1-2, s. 313-322. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.202100379. |
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@article{1850582, author = {Václavek, Tomáš and Foret, František}, article_number = {1-2}, doi = {http://dx.doi.org/10.1002/elps.202100379}, keywords = {bioanalysis; hemoglobin; mass spectrometry; microfluidics; single-cell analysis}, language = {eng}, issn = {0173-0835}, journal = {Electrophoresis}, title = {Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds}, url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elps.202100379}, volume = {44}, year = {2023} }
TY - JOUR ID - 1850582 AU - Václavek, Tomáš - Foret, František PY - 2023 TI - Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds JF - Electrophoresis VL - 44 IS - 1-2 SP - 313-322 EP - 313-322 PB - Verlag Chemie SN - 01730835 KW - bioanalysis KW - hemoglobin KW - mass spectrometry KW - microfluidics KW - single-cell analysis UR - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elps.202100379 N2 - Analysis of cellular composition and metabolism at a single-cell resolution allows gaining more information about complex relationships of cells within tissues or whole living organisms by resolving the variance stemming from the cellular heterogeneity. Mass spectrometry (MS) is a perfect analytical tool satisfying the demanding requirements of detecting and identifying compounds present in such ultralow-volume samples of high chemical complexity. However, the method of sampling and sample ionization is crucial in obtaining relevant information. In this work, we present a microfluidic sampling platform that integrates single-cell extraction from MS-incompatible media with electrical cell lysis and nanoESI-MS analysis of human erythrocytes. Hemoglobin alpha and beta chains (300 amol/cell) were successfully identified in mass spectra of single-erythrocyte lysates. ER -
VÁCLAVEK, Tomáš a František FORET. Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds. \textit{Electrophoresis}. Verlag Chemie, 2023, roč.~44, 1-2, s.~313-322. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.202100379.
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