Intervertebral disc degeneration is rescued by TGFβ/BMP
signaling modulation in an ex vivo filamin B mouse model

J 2022

Intervertebral disc degeneration is rescued by TGFβ/BMP signaling modulation in an ex vivo filamin B mouse model

ZIEBA, J., K. N. FORLENZA, K. HEARD, J. H. MARTIN, Michaela BOSÁKOVÁ et. al.

Basic information

Original name

Intervertebral disc degeneration is rescued by TGFβ/BMP signaling modulation in an ex vivo filamin B mouse model

Authors

ZIEBA, J., K. N. FORLENZA, K. HEARD, J. H. MARTIN, Michaela BOSÁKOVÁ (203 Czech Republic, belonging to the institution), D. H. COHN, S. P. ROBERTSON, Pavel KREJČÍ (203 Czech Republic, belonging to the institution) and D. KRAKOW (guarantor)

Edition

Bone research, London, SPRINGERNATURE, 2022, 2095-4700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30402 Technologies involving the manipulation of cells, tissues, organs or the whole organism

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 12.700

RIV identification code

RIV/00216224:14110/22:00125755

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41413-022-00200-5

UT WoS

000787774700001

Keywords in English

Spondylocarpotarsal syndrome; TGF beta/BMP signaling modulation; mouse model

Abstract

V originále

Spondylocarpotarsal syndrome (SCT) is a rare musculoskeletal disorder characterized by short stature and vertebral, carpal, and tarsal fusions resulting from biallelic nonsense mutations in the gene encoding filamin B (FLNB). Utilizing a FLNB knockout mouse, we showed that the vertebral fusions in SCT evolved from intervertebral disc (IVD) degeneration and ossification of the annulus fibrosus (AF), eventually leading to full trabecular bone formation. This resulted from alterations in the TGFβ/BMP signaling pathway that included increased canonical TGFβ and noncanonical BMP signaling. In this study, the role of FLNB in the TGFβ/BMP pathway was elucidated using in vitro, in vivo, and ex vivo treatment methodologies. The data demonstrated that FLNB interacts with inhibitory Smads 6 and 7 (i-Smads) to regulate TGFβ/BMP signaling and that loss of FLNB produces increased TGFβ receptor activity and decreased Smad 1 ubiquitination. Through the use of small molecule inhibitors in an ex vivo spine model, TGFβ/BMP signaling was modulated to design a targeted treatment for SCT and disc degeneration. Inhibition of canonical and noncanonical TGFβ/BMP pathway activity restored Flnb−/− IVD morphology. These most effective improvements resulted from specific inhibition of TGFβ and p38 signaling activation. FLNB acts as a bridge for TGFβ/BMP signaling crosstalk through i-Smads and is key for the critical balance in TGFβ/BMP signaling that maintains the IVD. These findings further our understanding of IVD biology and reveal new molecular targets for disc degeneration as well as congenital vertebral fusion disorders.

Citovat

ZIEBA, J., K. N. FORLENZA, K. HEARD, J. H. MARTIN, Michaela BOSÁKOVÁ, D. H. COHN, S. P. ROBERTSON, Pavel KREJČÍ and D. KRAKOW. Intervertebral disc degeneration is rescued by TGFβ/BMP signaling modulation in an ex vivo filamin B mouse model. Bone research. London: SPRINGERNATURE, 2022, vol. 10, No 1, p. 1-12. ISSN 2095-4700. Available from: https://dx.doi.org/10.1038/s41413-022-00200-5.

@article{1850798,
   author = {Zieba, J. and Forlenza, K. N. and Heard, K. and Martin, J. H. and Bosáková, Michaela and Cohn, D. H. and Robertson, S. P. and Krejčí, Pavel and Krakow, D.},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41413-022-00200-5},
   keywords = {Spondylocarpotarsal syndrome; TGF beta/BMP signaling modulation; mouse model},
   language = {eng},
   issn = {2095-4700},
   journal = {Bone research},
   title = {Intervertebral disc degeneration is rescued by TGFβ/BMP signaling modulation in an ex vivo filamin B mouse model},
   url = {https://www.nature.com/articles/s41413-022-00200-5},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 1850798
AU  - Zieba, J. - Forlenza, K. N. - Heard, K. - Martin, J. H. - Bosáková, Michaela - Cohn, D. H. - Robertson, S. P. - Krejčí, Pavel - Krakow, D.
PY  - 2022
TI  - Intervertebral disc degeneration is rescued by TGFβ/BMP signaling modulation in an ex vivo filamin B mouse model
JF  - Bone research
VL  - 10
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - SPRINGERNATURE
SN  - 20954700
KW  - Spondylocarpotarsal syndrome
KW  - TGF beta/BMP signaling modulation
KW  - mouse model
UR  - https://www.nature.com/articles/s41413-022-00200-5
N2  - Spondylocarpotarsal syndrome (SCT) is a rare musculoskeletal disorder characterized by short stature and vertebral, carpal, and tarsal fusions resulting from biallelic nonsense mutations in the gene encoding filamin B (FLNB). Utilizing a FLNB knockout mouse, we showed that the vertebral fusions in SCT evolved from intervertebral disc (IVD) degeneration and ossification of the annulus fibrosus (AF), eventually leading to full trabecular bone formation. This resulted from alterations in the TGFβ/BMP signaling pathway that included increased canonical TGFβ and noncanonical BMP signaling. In this study, the role of FLNB in the TGFβ/BMP pathway was elucidated using in vitro, in vivo, and ex vivo treatment methodologies. The data demonstrated that FLNB interacts with inhibitory Smads 6 and 7 (i-Smads) to regulate TGFβ/BMP signaling and that loss of FLNB produces increased TGFβ receptor activity and decreased Smad 1 ubiquitination. Through the use of small molecule inhibitors in an ex vivo spine model, TGFβ/BMP signaling was modulated to design a targeted treatment for SCT and disc degeneration. Inhibition of canonical and noncanonical TGFβ/BMP pathway activity restored Flnb−/− IVD morphology. These most effective improvements resulted from specific inhibition of TGFβ and p38 signaling activation. FLNB acts as a bridge for TGFβ/BMP signaling crosstalk through i-Smads and is key for the critical balance in TGFβ/BMP signaling that maintains the IVD. These findings further our understanding of IVD biology and reveal new molecular targets for disc degeneration as well as congenital vertebral fusion disorders.
ER  -

ZIEBA, J., K. N. FORLENZA, K. HEARD, J. H. MARTIN, Michaela BOSÁKOVÁ, D. H. COHN, S. P. ROBERTSON, Pavel KREJČÍ and D. KRAKOW. Intervertebral disc degeneration is rescued by TGFβ/BMP signaling modulation in an ex vivo filamin B mouse model. \textit{Bone research}. London: SPRINGERNATURE, 2022, vol.~10, No~1, p.~1-12. ISSN~2095-4700. Available from: https://dx.doi.org/10.1038/s41413-022-00200-5.

Detailed Information on Publication Record