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@article{1850799,
author = {Weissová, Kamila and Fafílek, Bohumil and Radaszkiewicz, Tomasz Witold and Çeliker, Canan and Macháčková, Petra and Čechová, Tamara and Šebestíková, Jana and Hampl, Aleš and Bryja, Vítězslav and Krejčí, Pavel and Bárta, Tomáš},
article_location = {COLD SPRING HARBOR},
article_number = {8},
doi = {http://dx.doi.org/10.26508/lsa.202201421},
keywords = {LuminoCell; luciferase-based reporters; real-time monitoring},
language = {eng},
issn = {2575-1077},
journal = {Life science alliance},
title = {LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters},
url = {https://www.life-science-alliance.org/content/5/8/e202201421},
volume = {5},
year = {2022}
}
TY - JOUR
ID - 1850799
AU - Weissová, Kamila - Fafílek, Bohumil - Radaszkiewicz, Tomasz Witold - Çeliker, Canan - Macháčková, Petra - Čechová, Tamara - Šebestíková, Jana - Hampl, Aleš - Bryja, Vítězslav - Krejčí, Pavel - Bárta, Tomáš
PY - 2022
TI - LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters
JF - Life science alliance
VL - 5
IS - 8
SP - 1-9
EP - 1-9
PB - LIFE SCIENCE ALLIANCE LLC
SN - 25751077
KW - LuminoCell
KW - luciferase-based reporters
KW - real-time monitoring
UR - https://www.life-science-alliance.org/content/5/8/e202201421
N2 - Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility of their use in different experimental conditions. Therefore, we aimed to develop a small, affordable luminometer allowing continuous measurement of luciferase activity, designed for inclusion into various kinds of tissue culture incubators. Here, we introduce LuminoCell—an open-source platform for the construction of an affordable, sensitive, and portable luminometer capable of real-time monitoring in-cell luciferase activity. The LuminoCell costs $40, requires less than 1 h to assemble, and it is capable of performing real-time sensitive detection of both magnitude and duration of the activity of major signalling pathways in cell cultures, including receptor tyrosine kinases (EGF and FGF), WNT/β-catenin, and NF-κB. In addition, we show that the LuminoCell is suitable to be used in cytotoxicity assays as well as for monitoring periodic circadian gene expression.
ER -
WEISSOVÁ, Kamila, Bohumil FAFÍLEK, Tomasz Witold RADASZKIEWICZ, Canan $\backslash$C CELIKER, Petra MACHÁČKOVÁ, Tamara ČECHOVÁ, Jana ŠEBESTÍKOVÁ, Aleš HAMPL, Vítězslav BRYJA, Pavel KREJČÍ and Tomáš BÁRTA. LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters. \textit{Life science alliance}. COLD SPRING HARBOR: LIFE SCIENCE ALLIANCE LLC, 2022, vol.~5, No~8, p.~1-9. ISSN~2575-1077. Available from: https://dx.doi.org/10.26508/lsa.202201421.
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