Detailed Information on Publication Record
2022
LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters
WEISSOVÁ, Kamila, Bohumil FAFÍLEK, Tomasz Witold RADASZKIEWICZ, Canan ÇELIKER, Petra MACHÁČKOVÁ et. al.Basic information
Original name
LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters
Authors
WEISSOVÁ, Kamila (203 Czech Republic, belonging to the institution), Bohumil FAFÍLEK (203 Czech Republic, belonging to the institution), Tomasz Witold RADASZKIEWICZ (616 Poland, belonging to the institution), Canan ÇELIKER (792 Turkey, belonging to the institution), Petra MACHÁČKOVÁ (203 Czech Republic, belonging to the institution), Tamara ČECHOVÁ (203 Czech Republic, belonging to the institution), Jana ŠEBESTÍKOVÁ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution), Vítězslav BRYJA (203 Czech Republic, belonging to the institution), Pavel KREJČÍ (203 Czech Republic, belonging to the institution) and Tomáš BÁRTA (203 Czech Republic, guarantor, belonging to the institution)
Edition
Life science alliance, COLD SPRING HARBOR, LIFE SCIENCE ALLIANCE LLC, 2022, 2575-1077
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10601 Cell biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.400
RIV identification code
RIV/00216224:14110/22:00125756
Organization unit
Faculty of Medicine
UT WoS
000793098700002
Keywords in English
LuminoCell; luciferase-based reporters; real-time monitoring
Tags
International impact, Reviewed
Změněno: 8/10/2024 10:28, Ing. Martina Blahová
Abstract
V originále
Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility of their use in different experimental conditions. Therefore, we aimed to develop a small, affordable luminometer allowing continuous measurement of luciferase activity, designed for inclusion into various kinds of tissue culture incubators. Here, we introduce LuminoCell—an open-source platform for the construction of an affordable, sensitive, and portable luminometer capable of real-time monitoring in-cell luciferase activity. The LuminoCell costs $40, requires less than 1 h to assemble, and it is capable of performing real-time sensitive detection of both magnitude and duration of the activity of major signalling pathways in cell cultures, including receptor tyrosine kinases (EGF and FGF), WNT/β-catenin, and NF-κB. In addition, we show that the LuminoCell is suitable to be used in cytotoxicity assays as well as for monitoring periodic circadian gene expression.
Links
| GA19-20123S, research and development project |
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| GA21-08182S, research and development project |
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| GF21-26400K, research and development project |
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| GX19-28347X, research and development project |
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| LM2018129, research and development project |
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| MUNI/A/1398/2021, interní kód MU |
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| MUNI/A/1418/2021, interní kód MU |
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| MUNI/G/1391/2018, interní kód MU |
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| 90129, large research infrastructures |
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