J 2022

LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters

WEISSOVÁ, Kamila, Bohumil FAFÍLEK, Tomasz Witold RADASZKIEWICZ, Canan ÇELIKER, Petra MACHÁČKOVÁ et. al.

Basic information

Original name

LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters

Authors

WEISSOVÁ, Kamila (203 Czech Republic, belonging to the institution), Bohumil FAFÍLEK (203 Czech Republic, belonging to the institution), Tomasz Witold RADASZKIEWICZ (616 Poland, belonging to the institution), Canan ÇELIKER (792 Turkey, belonging to the institution), Petra MACHÁČKOVÁ (203 Czech Republic, belonging to the institution), Tamara ČECHOVÁ (203 Czech Republic, belonging to the institution), Jana ŠEBESTÍKOVÁ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution), Vítězslav BRYJA (203 Czech Republic, belonging to the institution), Pavel KREJČÍ (203 Czech Republic, belonging to the institution) and Tomáš BÁRTA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Life science alliance, COLD SPRING HARBOR, LIFE SCIENCE ALLIANCE LLC, 2022, 2575-1077

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.400

RIV identification code

RIV/00216224:14110/22:00125756

Organization unit

Faculty of Medicine

UT WoS

000793098700002

Keywords in English

LuminoCell; luciferase-based reporters; real-time monitoring

Tags

International impact, Reviewed
Změněno: 24/10/2024 11:04, Mgr. Adéla Pešková

Abstract

V originále

Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility of their use in different experimental conditions. Therefore, we aimed to develop a small, affordable luminometer allowing continuous measurement of luciferase activity, designed for inclusion into various kinds of tissue culture incubators. Here, we introduce LuminoCell—an open-source platform for the construction of an affordable, sensitive, and portable luminometer capable of real-time monitoring in-cell luciferase activity. The LuminoCell costs $40, requires less than 1 h to assemble, and it is capable of performing real-time sensitive detection of both magnitude and duration of the activity of major signalling pathways in cell cultures, including receptor tyrosine kinases (EGF and FGF), WNT/β-catenin, and NF-κB. In addition, we show that the LuminoCell is suitable to be used in cytotoxicity assays as well as for monitoring periodic circadian gene expression.

Links

GA19-20123S, research and development project
Name: Regulace časného vývoje savčí končetiny pomocí nestabilních morfogenů z rodiny FGF (Acronym: Regulace časného vývoje savčí končetiny)
Investor: Czech Science Foundation
GA21-08182S, research and development project
Name: Retinální organoidy: Zkoumání účinků fotostimulace na lidskou sítnici
Investor: Czech Science Foundation
GF21-26400K, research and development project
Name: Vztah struktury a funkce v signálováni fibroblastových růstových faktorů
Investor: Czech Science Foundation, Lead Agency
GX19-28347X, research and development project
Name: Molekulární a funkční analýza biologie kasein kinázy 1
Investor: Czech Science Foundation
LM2018129, research and development project
Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1398/2021, interní kód MU
Name: Zdroje pro tkáňové inženýrství 12 (Acronym: TissueEng 12)
Investor: Masaryk University
MUNI/A/1418/2021, interní kód MU
Name: Biomedicínské vědy II (Acronym: BIOMED)
Investor: Masaryk University
MUNI/G/1391/2018, interní kód MU
Name: At the Cross-road of Magnetic Fields and Light: A New Perspective of Cell Clock Control.
Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects