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@proceedings{1851157,
author = {Souralová, Tereza and Ventruba, Pavel and Ješeta, Michal and Řeháková, Daniela and Hampl, Aleš and Koutná, Irena},
booktitle = {SY-STEM 2022, Vienna, Austria},
language = {eng},
title = {Establishment of human embryonic stem cells for stem-cell therapies},
url = {https://www.oeaw.ac.at/imba/seminars-events/sy-stem},
year = {2022}
}
TY - CONF
ID - 1851157
AU - Souralová, Tereza - Ventruba, Pavel - Ješeta, Michal - Řeháková, Daniela - Hampl, Aleš - Koutná, Irena
PY - 2022
TI - Establishment of human embryonic stem cells for stem-cell therapies
UR - https://www.oeaw.ac.at/imba/seminars-events/sy-stem
N2 - Human embryonic stem cells are able to differentiate into every cell type in the human body making them a tremendous cell source for regenerative medicine. However, the production of human embryonic stem cell lines for clinical use is challenging as clean rooms, highly-qualified personnel, standard operating procedures for both manufacture and quality control are required. Here we present the derivation of clinical-grade hESC lines in cooperation with the Centre of assisted reproduction - University Hospital Brno which provided 6-day old blastocysts. Laminin 521 in combination with Nutristem, Human serum albumin, and ECadherin was used for the mechanical derivation. Human embryonic stem cells were cultured on laminin 521 in a Nutristem medium. The derivation itself and subsequent culture are xeno-free, feeder-free, and fulfill standards of current good manufacturing practices. In-depth quality control provides essential information about the safety, pluripotency, and differentiation potential of lines. Presented clinical-grade hESC lines were established according to state-of-the-art technology that makes them excellent cell source for stem cell-based therapies.
ER -
SOURALOVÁ, Tereza, Pavel VENTRUBA, Michal JEŠETA, Daniela ŘEHÁKOVÁ, Aleš HAMPL and Irena KOUTNÁ. Establishment of human embryonic stem cells for stem-cell therapies. In \textit{SY-STEM 2022, Vienna, Austria}. 2022.
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