J 2022

Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

ŠVEC, Pavel, Stanislava KRÁLOVÁ, Eva STAŇKOVÁ, Pavla HOLOCHOVÁ, Karel SEDLÁŘ et. al.

Basic information

Original name

Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

Authors

ŠVEC, Pavel (203 Czech Republic, belonging to the institution), Stanislava KRÁLOVÁ (703 Slovakia, guarantor, belonging to the institution), Eva STAŇKOVÁ (203 Czech Republic, belonging to the institution), Pavla HOLOCHOVÁ (203 Czech Republic, belonging to the institution), Karel SEDLÁŘ, Sylva KOUDELKOVÁ (203 Czech Republic, belonging to the institution), Daniel KRSEK, Jakub GRZESIAK, Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Peter VÁCZI (703 Slovakia, belonging to the institution), Urvashi SAH, Vipin GUPTA, Utkarsh SOOD, Rup LAL, Suresh KORPOLE and Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution)

Edition

International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2022, 1466-5026

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.800

RIV identification code

RIV/00216224:14310/22:00125779

Organization unit

Faculty of Science

DOI

UT WoS

000800144400027

Keywords in English

Pedobacter fastidiosus sp. nov.; proglacial stream; glacier; Antarctica; taxonomy

Tags

Tags

International impact, Reviewed
Změněno: 2/11/2024 21:08, Ing. Martina Blahová

Abstract

V originále

Strains P8930T and 478 were isolated from Antarctic glaciers located on James Ross Island and King George Island, respectively. They comprised Gram-stain-negative short rod-shaped cells forming pink pigmented colonies and exhibited identical 16S rRNA gene sequences and highly similar MALDI TOF mass spectra, and hence were assigned as representatives of the same species. Phylogenetic analysis based on 16S rRNA gene sequences assigned both isolates to the genus Pedobacter and showed Pedobacter frigidisoli and Pedobacter terrae to be their closest phylogenetic neighbours, with 97.4 and 97.2 % 16S rRNA gene sequence similarities, respectively. These low similarity values were below the threshold similarity value of 98.7%, confirming the delineation of a new bacterial species. Further genomic characterization included whole-genome sequencing accompanied by average nucleotide identity (ANI) and digital DNA–DNA hybridization calculations, and characterization of the genome features. The ANI values between P8930T and P. frigidisoli RP-3-11T and P. terrae DSM 17933T were 79.7 and 77.6 %, respectively, and the value between P. frigidisoli RP-3-11T and P. terrae DSM 17933T was 77.7 %, clearly demonstrating the phylogenetic distance and the novelty of strain P8930T. Further characterization included analysis of cellular fatty acids, quinones and polar lipids, and comprehensive biotyping. All the obtained results proved the separation of strains P8930T and 478 from the other validly named Pedobacter species, and confirmed that they represent a new species for which the name Pedobacter fastidiosus sp. nov. is proposed. The type strain is P8930T (=CCM 8938T=LMG 32098T).

Links

EF16_013/0001708, research and development project
Name: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (CzechPolar2)
EF18_046/0015974, research and development project
Name: Modernizace České infrastruktury pro integrativní strukturní biologii
LM2015078, research and development project
Name: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)
Investor: Ministry of Education, Youth and Sports of the CR
90127, large research infrastructures
Name: CIISB II