Detailed Information on Publication Record
2022
mRNA Subtype of Cancer-Associated Fibroblasts Significantly Affects Key Characteristics of Head and Neck Cancer Cells
PELTANOVÁ, Barbora, Hana HOLCOVÁ POLANSKÁ, Martina RAUDENSKÁ, Jan BALVAN, Jiří NAVRÁTIL et. al.Basic information
Original name
mRNA Subtype of Cancer-Associated Fibroblasts Significantly Affects Key Characteristics of Head and Neck Cancer Cells
Authors
PELTANOVÁ, Barbora (203 Czech Republic, belonging to the institution), Hana HOLCOVÁ POLANSKÁ (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Jan BALVAN (203 Czech Republic, belonging to the institution), Jiří NAVRÁTIL (203 Czech Republic, belonging to the institution), Tomáš VIČAR (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Barbora ČECHOVÁ (203 Czech Republic, belonging to the institution), Martin KRÄTER, Jochen GUCK, David KALFEŘT (203 Czech Republic), Marek GREGA (203 Czech Republic), Jan PLZÁK (203 Czech Republic), Jan BETKA (203 Czech Republic) and Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution)
Edition
Cancers, Basel, MDPI, 2022, 2072-6694
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30204 Oncology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.200
RIV identification code
RIV/00216224:14110/22:00125782
Organization unit
Faculty of Medicine
UT WoS
000794813300001
Keywords in English
cancer; HNSCC; cancer-associated fibroblasts; tumour microenvironment; cell stiffness
Tags
International impact, Reviewed
Změněno: 19/7/2022 10:45, Mgr. Tereza Miškechová
Abstract
V originále
Head and neck squamous cell carcinomas (HNSCC) belong among severe and highly complex malignant diseases showing a high level of heterogeneity and consequently also a variance in therapeutic response, regardless of clinical stage. Our study implies that the progression of HNSCC may be supported by cancer-associated fibroblasts (CAFs) in the tumour microenvironment (TME) and the heterogeneity of this disease may lie in the level of cooperation between CAFs and epithelial cancer cells, as communication between CAFs and epithelial cancer cells seems to be a key factor for the sustained growth of the tumour mass. In this study, we investigated how CAFs derived from tumours of different mRNA subtypes influence the proliferation of cancer cells and their metabolic and biomechanical reprogramming. We also investigated the clinicopathological significance of the expression of these metabolism-related genes in tissue samples of HNSCC patients to identify a possible gene signature typical for HNSCC progression. We found that the right kind of cooperation between cancer cells and CAFs is needed for tumour growth and progression, and only specific mRNA subtypes can support the growth of primary cancer cells or metastases. Specifically, during coculture, cancer cell colony supporting effect and effect of CAFs on cell stiffness of cancer cells are driven by the mRNA subtype of the tumour from which the CAFs are derived. The degree of colony-forming support is reflected in cancer cell glycolysis levels and lactate shuttle-related transporters.
Links
| FW03010186, research and development project |
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| GA18-03978S, research and development project |
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| LM2018129, research and development project |
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| MUNI/A/1133/2021, interní kód MU |
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| MUNI/A/1391/2021, interní kód MU |
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| NV18-08-00229, research and development project |
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