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@article{1852763,
author = {Kubatka, Peter and Kapinová, Andrea and Kello, Martin and Kružliak, Peter and Kajo, Karol and Výbohová, Desanka and Mahmood, Silvia and Murin, Radovan and Viera, Tischlerová and Mojžiš, Ján and Zulli, Anthony and Péč, Martin and Adamkov, Marián and Kassayová, Monika and Bojková, Bianka and Stollárová, Nadežda and Dobrota, Dušan},
article_location = {HEIDELBERG},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s00394-015-0910-5},
keywords = {Angiogenesis; Apoptosis; Cell proliferation; Fruit polyphenols; Mammary carcinogenesis; MCF-7; Rat},
language = {eng},
issn = {1436-6207},
journal = {EUROPEAN JOURNAL OF NUTRITION},
title = {Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer},
url = {https://link.springer.com/article/10.1007/s00394-015-0910-5},
volume = {55},
year = {2016}
}
TY - JOUR
ID - 1852763
AU - Kubatka, Peter - Kapinová, Andrea - Kello, Martin - Kružliak, Peter - Kajo, Karol - Výbohová, Desanka - Mahmood, Silvia - Murin, Radovan - Viera, Tischlerová - Mojžiš, Ján - Zulli, Anthony - Péč, Martin - Adamkov, Marián - Kassayová, Monika - Bojková, Bianka - Stollárová, Nadežda - Dobrota, Dušan
PY - 2016
TI - Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer
JF - EUROPEAN JOURNAL OF NUTRITION
VL - 55
IS - 3
SP - 955-965
EP - 955-965
PB - SPRINGER HEIDELBERG
SN - 14366207
KW - Angiogenesis
KW - Apoptosis
KW - Cell proliferation
KW - Fruit polyphenols
KW - Mammary carcinogenesis
KW - MCF-7
KW - Rat
UR - https://link.springer.com/article/10.1007/s00394-015-0910-5
N2 - Purpose Fruit and vegetable intake is inversely correlated with cancer; thus, it is proposed that an extract of phytochemicals as present in whole fruits, vegetables, or grains may have anti-carcinogenic properties. Thus, the anti-tumour effects of fruit peel polyphenols (Flavin7) in the chemoprevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis in female rats were evaluated. Methods Lyophilized substance of Flavin7 (F7) was administered at two concentrations of 0.3 and 3 % through diet. The experiment was terminated 14 weeks after carcinogen administration, and mammary tumours were removed and prepared for histopathological and immunohistochemical analysis. In addition, using an in vitro cytotoxicity assay, apoptosis and proliferation after F7 treatment in human breast adenocarcinoma (MCF-7) cells were performed. Results High-dose F7 suppressed tumour frequency by 58 % (P < 0.001), tumour incidence by 24 % (P < 0.05), and lengthened latency by 8 days (P > 0.05) in comparison with the control rats, whereas lower dose of F7 was less effective. Histopathological analysis of tumours showed significant decrease in the ratio of high-/low-grade carcinomas after high-dose F7 treatment. Immunohistochemical analysis of rat carcinoma cells in vivo found a significant increase in caspase-3 expression and significant decrease in Bcl-2, Ki67, and VEGFR-2 expression in the high-dose group. Both doses demonstrated significant positive effects on plasma lipid metabolism in rats. F7 significantly decreased survival of MCF-7 cells in vitro in MTT assay by dose- and time-dependent manner compared to control. F7 prevented cell cycle progression by significant enrichment in G1 cell populations. Incubation with F7 showed significant increase in the percentage of annexin V-/PI-positive MCF-7 cells and DNA fragmentation. Conclusions Our results reveal a substantial tumour-suppressive effect of F7 in the breast cancer model. We propose that the effects of phytochemicals present in this fruit extract are responsible for observed potent anti-cancer activities.
ER -
KUBATKA, Peter, Andrea KAPINOVÁ, Martin KELLO, Peter KRUŽLIAK, Karol KAJO, Desanka VÝBOHOVÁ, Silvia MAHMOOD, Radovan MURIN, Tischlerová VIERA, Ján MOJŽIŠ, Anthony ZULLI, Martin PÉČ, Marián ADAMKOV, Monika KASSAYOVÁ, Bianka BOJKOVÁ, Nadežda STOLLÁROVÁ and Dušan DOBROTA. Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer. \textit{EUROPEAN JOURNAL OF NUTRITION}. HEIDELBERG: SPRINGER HEIDELBERG, 2016, vol.~55, No~3, p.~955-965. ISSN~1436-6207. Available from: https://dx.doi.org/10.1007/s00394-015-0910-5.
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