ENZYME LINKED RECEPTOR PROTEIN SIGNALING PATHWAY IS ONE OF THE
ONTOLOGY GROUPS THAT ARE HIGHLY UP-REGULATED IN PORCINE OOCYTES
BEFORE IN VITRO MATURATION

J 2018

ENZYME LINKED RECEPTOR PROTEIN SIGNALING PATHWAY IS ONE OF THE ONTOLOGY GROUPS THAT ARE HIGHLY UP-REGULATED IN PORCINE OOCYTES BEFORE IN VITRO MATURATION

BORYS, S., M. BRAZERT, M. JANKOWSKI, I. KOCHEROVA, P. CELICHOWSKI et. al.

Basic information

Original name

ENZYME LINKED RECEPTOR PROTEIN SIGNALING PATHWAY IS ONE OF THE ONTOLOGY GROUPS THAT ARE HIGHLY UP-REGULATED IN PORCINE OOCYTES BEFORE IN VITRO MATURATION

Authors

BORYS, S., M. BRAZERT, M. JANKOWSKI, I. KOCHEROVA, P. CELICHOWSKI, M. J. NAWROCKI, W. KRANC, A. BRYJA, M. KULUS, Michal JEŠETA (203 Czech Republic, belonging to the institution), W. PIENKOWSKI, A. BREBOROWICZ, D. BUKOWSKA, P. ANTOSIK, L. PAWELCZYK, M. T. SKOWRONSKI, K. P. BRUSSOW, M. BRUSKA, M. ZABEL, M. NOWICKI, K. OZEGOWSKA and B. KEMPISTY (guarantor)

Edition

Journal of biological regulators and homeostatic agents, Silva Marina, Biolife SAS, 2018, 0393-974X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.558

RIV identification code

RIV/00216224:14110/18:00124603

Organization unit

Faculty of Medicine

UT WoS

000449211700005

Keywords in English

pig; oocytes; microarray assays; in vitro maturation (IVM)

Abstract

V originále

Before being able to fully participate in the processes associated with its function as a female gamete, the oocyte needs to undergo a range of changes to achieve its mature form. These morphological, biochemical and metabolomic processes are induced by the somatic tissues surrounding the oocyte, through the expression of specific transcription and growth factors. The maturation of the oocyte is highly important for the proceedings that lead to successful fertilization, early embryonic development and implantation. Domestic pigs were used as models for our study, with the cumulus-oocyte complexes obtained from the ovaries that were recovered at slaughter. After shedding of the cumulus, oocytes were assessed with BCB test, with the viable ones chosen to undergo in vitro maturation. With the use of expression microarrays, we analyzed gene expression before and after IVM and detected major changes in both genes that were proven to be associated with oocyte maturation before (FOS, VEGFA, CHRDL1, TGFBR3, FST, INSR, ID1, TXNIP, SMAD4, MAP3K1, EIF2AK3 and KIT) and genes not previously linked with reproduction associated processes (MYO1E,PHII; KLF10 and SHOC2). All the genes were briefly described, with consideration of possible involvement of the newly discovered elements of the transcriptome in the process of oocyte maturation.

Citovat

BORYS, S., M. BRAZERT, M. JANKOWSKI, I. KOCHEROVA, P. CELICHOWSKI, M. J. NAWROCKI, W. KRANC, A. BRYJA, M. KULUS, Michal JEŠETA, W. PIENKOWSKI, A. BREBOROWICZ, D. BUKOWSKA, P. ANTOSIK, L. PAWELCZYK, M. T. SKOWRONSKI, K. P. BRUSSOW, M. BRUSKA, M. ZABEL, M. NOWICKI, K. OZEGOWSKA and B. KEMPISTY. ENZYME LINKED RECEPTOR PROTEIN SIGNALING PATHWAY IS ONE OF THE ONTOLOGY GROUPS THAT ARE HIGHLY UP-REGULATED IN PORCINE OOCYTES BEFORE IN VITRO MATURATION. Journal of biological regulators and homeostatic agents. Silva Marina: Biolife SAS, 2018, vol. 32, No 5, p. 1089-1103. ISSN 0393-974X.

@article{1853560,
   author = {Borys, S. and Brazert, M. and Jankowski, M. and Kocherova, I. and Celichowski, P. and Nawrocki, M. J. and Kranc, W. and Bryja, A. and Kulus, M. and Ješeta, Michal and Pienkowski, W. and Breborowicz, A. and Bukowska, D. and Antosik, P. and Pawelczyk, L. and Skowronski, M. T. and Brussow, K. P. and Bruska, M. and Zabel, M. and Nowicki, M. and Ozegowska, K. and Kempisty, B.},
   article_location = {Silva Marina},
   article_number = {5},
   keywords = {pig; oocytes; microarray assays; in vitro maturation (IVM)},
   language = {eng},
   issn = {0393-974X},
   journal = {Journal of biological regulators and homeostatic agents},
   title = {ENZYME LINKED RECEPTOR PROTEIN SIGNALING PATHWAY IS ONE OF THE ONTOLOGY GROUPS THAT ARE HIGHLY UP-REGULATED IN PORCINE OOCYTES BEFORE IN VITRO MATURATION},
   url = {https://pubmed.ncbi.nlm.nih.gov/30334402/},
   volume = {32},
   year = {2018}
}

TY  - JOUR
ID  - 1853560
AU  - Borys, S. - Brazert, M. - Jankowski, M. - Kocherova, I. - Celichowski, P. - Nawrocki, M. J. - Kranc, W. - Bryja, A. - Kulus, M. - Ješeta, Michal - Pienkowski, W. - Breborowicz, A. - Bukowska, D. - Antosik, P. - Pawelczyk, L. - Skowronski, M. T. - Brussow, K. P. - Bruska, M. - Zabel, M. - Nowicki, M. - Ozegowska, K. - Kempisty, B.
PY  - 2018
TI  - ENZYME LINKED RECEPTOR PROTEIN SIGNALING PATHWAY IS ONE OF THE ONTOLOGY GROUPS THAT ARE HIGHLY UP-REGULATED IN PORCINE OOCYTES BEFORE IN VITRO MATURATION
JF  - Journal of biological regulators and homeostatic agents
VL  - 32
IS  - 5
SP  - 1089-1103
EP  - 1089-1103
PB  - Biolife SAS
SN  - 0393974X
KW  - pig
KW  - oocytes
KW  - microarray assays
KW  - in vitro maturation (IVM)
UR  - https://pubmed.ncbi.nlm.nih.gov/30334402/
N2  - Before being able to fully participate in the processes associated with its function as a female gamete, the oocyte needs to undergo a range of changes to achieve its mature form. These morphological, biochemical and metabolomic processes are induced by the somatic tissues surrounding the oocyte, through the expression of specific transcription and growth factors. The maturation of the oocyte is highly important for the proceedings that lead to successful fertilization, early embryonic development and implantation. Domestic pigs were used as models for our study, with the cumulus-oocyte complexes obtained from the ovaries that were recovered at slaughter. After shedding of the cumulus, oocytes were assessed with BCB test, with the viable ones chosen to undergo in vitro maturation. With the use of expression microarrays, we analyzed gene expression before and after IVM and detected major changes in both genes that were proven to be associated with oocyte maturation before (FOS, VEGFA, CHRDL1, TGFBR3, FST, INSR, ID1, TXNIP, SMAD4, MAP3K1, EIF2AK3 and KIT) and genes not previously linked with reproduction associated processes (MYO1E,PHII; KLF10 and SHOC2). All the genes were briefly described, with consideration of possible involvement of the newly discovered elements of the transcriptome in the process of oocyte maturation.
ER  -

BORYS, S., M. BRAZERT, M. JANKOWSKI, I. KOCHEROVA, P. CELICHOWSKI, M. J. NAWROCKI, W. KRANC, A. BRYJA, M. KULUS, Michal JEŠETA, W. PIENKOWSKI, A. BREBOROWICZ, D. BUKOWSKA, P. ANTOSIK, L. PAWELCZYK, M. T. SKOWRONSKI, K. P. BRUSSOW, M. BRUSKA, M. ZABEL, M. NOWICKI, K. OZEGOWSKA and B. KEMPISTY. ENZYME LINKED RECEPTOR PROTEIN SIGNALING PATHWAY IS ONE OF THE ONTOLOGY GROUPS THAT ARE HIGHLY UP-REGULATED IN PORCINE OOCYTES BEFORE IN VITRO MATURATION. \textit{Journal of biological regulators and homeostatic agents}. Silva Marina: Biolife SAS, 2018, vol.~32, No~5, p.~1089-1103. ISSN~0393-974X.

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