Metagenomic research of the ancient human dental calculus as a
potential source for the study of rare infectious diseases

a 2022

Metagenomic research of the ancient human dental calculus as a potential source for the study of rare infectious diseases

CHOCHOLOVÁ, Eva, Eva DROZDOVÁ, Dana FIALOVÁ, Martin ČERNÝ, Barbora ZWINSOVÁ et. al.

Basic information

Original name

Metagenomic research of the ancient human dental calculus as a potential source for the study of rare infectious diseases

Authors

CHOCHOLOVÁ, Eva (203 Czech Republic, guarantor, belonging to the institution), Eva DROZDOVÁ (203 Czech Republic, belonging to the institution), Dana FIALOVÁ (203 Czech Republic, belonging to the institution), Martin ČERNÝ (203 Czech Republic, belonging to the institution), Barbora ZWINSOVÁ (203 Czech Republic, belonging to the institution), Petra VÍDEŇSKÁ (203 Czech Republic, belonging to the institution), Kristýna BRZOBOHATÁ (203 Czech Republic, belonging to the institution) and Kateřina NOVOTNÁ (203 Czech Republic, belonging to the institution)

Edition

The Biomania Student Scientific Meeting 2022, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/22:00125840

Organization unit

Faculty of Science

ISBN

978-80-280-0040-0

Keywords (in Czech)

starobylá DNA; aDNA; vzácná onemocnění; vzácná infekční onemocnění; patogen; zubní kámen; metagenomika

Keywords in English

ancient DNA; aDNA; rare disease; rare infectious disease; pathogen; dental calculus; metagenomics

Abstract

V originále

Ancient human dental calculus has been intensively studied in recent years by diverse molecular methods. It was found to be a rich and important source for detailed study of past populations, as well as their pathogens. This study presents the results of metagenomic research by sequencing 16S rRNA gene from bacteria trapped inside tartar and suggests the possibility of using such an approach to screen samples for rare infectious diseases. Dental calculus samples came from populations of four different periods: Copper Age (Funnelbeaker culture), Bronze Age (Unetice), Middle Ages (Great Moravia), and early modern period, which provides a comparison. Genera found include Nocardia, Haemophilus, Bartonella, Shigella, or Neisseria. Applied approach is recommended as a robust screening method. All laboratory procedures were carried out in a dedicated ancient biomolecule facility of the Laboratory of Biological and Molecular Anthropology.

Links

MUNI/A/1325/2021, interní kód MU

Name: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10 (Acronym: MBG10)

Investor: Masaryk University

Citovat

CHOCHOLOVÁ, Eva, Eva DROZDOVÁ, Dana FIALOVÁ, Martin ČERNÝ, Barbora ZWINSOVÁ, Petra VÍDEŇSKÁ, Kristýna BRZOBOHATÁ and Kateřina NOVOTNÁ. Metagenomic research of the ancient human dental calculus as a potential source for the study of rare infectious diseases. In The Biomania Student Scientific Meeting 2022. 2022. ISBN 978-80-280-0040-0.

@proceedings{1854279,
   author = {Chocholová, Eva and Drozdová, Eva and Fialová, Dana and Černý, Martin and Zwinsová, Barbora and Vídeňská, Petra and Brzobohatá, Kristýna and Novotná, Kateřina},
   booktitle = {The Biomania Student Scientific Meeting 2022},
   keywords = {ancient DNA; aDNA; rare disease; rare infectious disease; pathogen; dental calculus; metagenomics},
   language = {eng},
   isbn = {978-80-280-0040-0},
   title = {Metagenomic research of the ancient human dental calculus as a potential source for the study of rare infectious diseases},
   year = {2022}
}

TY  - CONF
ID  - 1854279
AU  - Chocholová, Eva - Drozdová, Eva - Fialová, Dana - Černý, Martin - Zwinsová, Barbora - Vídeňská, Petra - Brzobohatá, Kristýna - Novotná, Kateřina
PY  - 2022
TI  - Metagenomic research of the ancient human dental calculus as a potential source for the study of rare infectious diseases
SN  - 9788028000400
KW  - ancient DNA
KW  - aDNA
KW  - rare disease
KW  - rare infectious disease
KW  - pathogen
KW  - dental calculus
KW  - metagenomics
N2  - Ancient human dental calculus has been intensively studied in recent years by diverse molecular methods. It was found to be a rich and important source for detailed study of past populations, as well as their pathogens. This study presents the results of metagenomic research by sequencing 16S rRNA gene from bacteria trapped inside tartar and suggests the possibility of using such an approach to screen samples for rare infectious diseases. Dental calculus samples came from populations of four different periods: Copper Age (Funnelbeaker culture), Bronze Age (Unetice), Middle Ages (Great Moravia), and early modern period, which provides a comparison. Genera found include Nocardia, Haemophilus, Bartonella, Shigella, or Neisseria. Applied approach is recommended as a robust screening method. All laboratory procedures were carried out in a dedicated ancient biomolecule facility of the Laboratory of Biological and Molecular Anthropology.
ER  -

CHOCHOLOVÁ, Eva, Eva DROZDOVÁ, Dana FIALOVÁ, Martin ČERNÝ, Barbora ZWINSOVÁ, Petra VÍDEŇSKÁ, Kristýna BRZOBOHATÁ and Kateřina NOVOTNÁ. Metagenomic research of the ancient human dental calculus as a potential source for the study of rare infectious diseases. In \textit{The Biomania Student Scientific Meeting 2022}. 2022. ISBN~978-80-280-0040-0.

Detailed Information on Publication Record