Detailed Information on Publication Record
2022
Cryptic inhibitory regions nearby activation domains
KNIGHT, Andrea and Martin PISKÁČEKBasic information
Original name
Cryptic inhibitory regions nearby activation domains
Authors
KNIGHT, Andrea (203 Czech Republic, belonging to the institution) and Martin PISKÁČEK (40 Austria, guarantor, belonging to the institution)
Edition
Biochimie, ISSY-LES-MOULINEAUX, Elsevier, 2022, 0300-9084
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30105 Physiology
Country of publisher
France
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.900
RIV identification code
RIV/00216224:14110/22:00129669
Organization unit
Faculty of Medicine
UT WoS
000806357600003
Keywords in English
Transcription; Gal4; MED15; 9aaTAD
Změněno: 19/7/2022 09:20, Mgr. Tereza Miškechová
Abstract
V originále
Previously, the Nine amino acid TransActivation Domain (9aaTAD) was identified in the Gal4 region 862–870 (DDVYNYLFD). Here, we identified 9aaTADs in the distal Gal4 orthologs by our prediction algorithm and found their conservation in the family. The 9aaTAD function as strong activators was demonstrated. We identified adjacent Gal4 region 871–811 (DEDTPPNPKKE) as a natural 9aaTAD inhibitory domain located at the extreme Gal4 terminus. Moreover, we identified conserved Gal4 region 172–185 (FDWSEEDDMSDGLP), which was capable to reverse the 9aaTAD inhibition. In conclusion, our results uncover the existence of the cryptic inhibitory domains, which need to be carefully implemented in all functional studies with transcription factors to avoid incorrect conclusions.
Links
| NV19-05-00410, research and development project |
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