The ArsH Protein Product of the Paracoccus denitrificans ars
Operon Has an Activity of Organoarsenic Reductase and Is
Regulated by a Redox-Responsive Repressor

J 2022

The ArsH Protein Product of the Paracoccus denitrificans ars Operon Has an Activity of Organoarsenic Reductase and Is Regulated by a Redox-Responsive Repressor

SEDLÁČEK, Vojtěch, Martin KRYL and Igor KUČERA

Basic information

Original name

The ArsH Protein Product of the Paracoccus denitrificans ars Operon Has an Activity of Organoarsenic Reductase and Is Regulated by a Redox-Responsive Repressor

Authors

SEDLÁČEK, Vojtěch (203 Czech Republic, belonging to the institution), Martin KRYL (203 Czech Republic, belonging to the institution) and Igor KUČERA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Antioxidants, Basel, MDPI, 2022, 2076-3921

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.000

RIV identification code

RIV/00216224:14310/22:00125977

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/antiox11050902

UT WoS

000803523700001

Keywords in English

FMN; NADPH; organoarsenicals; Paracoccus denitrificans; redox-responsive repressor

Abstract

V originále

Paracoccus denitrificans ArsH is encoded by two identical genes located in two distinct putative arsenic resistance (ars) operons. Escherichia coli-produced recombinant N-His6-ArsH was characterized both structurally and kinetically. The X-ray structure of ArsH revealed a flavodoxin-like domain and motifs for the binding of flavin mononucleotide (FMN) and reduced nicotinamide adenine dinucleotide phosphate (NADPH). The protein catalyzed FMN reduction by NADPH via ternary complex mechanism. At a fixed saturating FMN concentration, it acted as an NADPH-dependent organoarsenic reductase displaying ping-pong kinetics. A 1:1 enzymatic reaction of phenylarsonic acid with the reduced form of FMN (FMNH2) and formation of phenylarsonous acid were observed. Growth experiments with P. denitrificans and E. coli revealed increased toxicity of phenylarsonic acid to cells expressing arsH, which may be related to in vivo conversion of pentavalent As to more toxic trivalent form. ArsH expression was upregulated not only by arsenite, but also by redox-active agents paraquat, tert-butyl hydroperoxide and diamide. A crucial role is played by the homodimeric transcriptional repressor ArsR, which was shown in in vitro experiments to monomerize and release from the DNA-target site. Collectively, our results establish ArsH as responsible for enhancement of organo-As(V) toxicity and demonstrate redox control of ars operon.

Links

GA16-18476S, research and development project

Name: Oxidační stres u denitrifikačních baktérií: objasnění funkce zúčastněných proteinů a možných dopadů na životní prostředí

Investor: Czech Science Foundation

MUNI/A/1604/2020, interní kód MU

Name: Podpora biochemického výzkumu v roce 2021

Investor: Masaryk University

Citovat

SEDLÁČEK, Vojtěch, Martin KRYL and Igor KUČERA. The ArsH Protein Product of the Paracoccus denitrificans ars Operon Has an Activity of Organoarsenic Reductase and Is Regulated by a Redox-Responsive Repressor. Antioxidants. Basel: MDPI, 2022, vol. 11, No 5, p. 1-18. ISSN 2076-3921. Available from: https://dx.doi.org/10.3390/antiox11050902.

@article{1859238,
   author = {Sedláček, Vojtěch and Kryl, Martin and Kučera, Igor},
   article_location = {Basel},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/antiox11050902},
   keywords = {FMN; NADPH; organoarsenicals; Paracoccus denitrificans; redox-responsive repressor},
   language = {eng},
   issn = {2076-3921},
   journal = {Antioxidants},
   title = {The ArsH Protein Product of the Paracoccus denitrificans ars Operon Has an Activity of Organoarsenic Reductase and Is Regulated by a Redox-Responsive Repressor},
   url = {https://www.mdpi.com/2076-3921/11/5/902},
   volume = {11},
   year = {2022}
}

TY  - JOUR
ID  - 1859238
AU  - Sedláček, Vojtěch - Kryl, Martin - Kučera, Igor
PY  - 2022
TI  - The ArsH Protein Product of the Paracoccus denitrificans ars Operon Has an Activity of Organoarsenic Reductase and Is Regulated by a Redox-Responsive Repressor
JF  - Antioxidants
VL  - 11
IS  - 5
SP  - 1-18
EP  - 1-18
PB  - MDPI
SN  - 20763921
KW  - FMN
KW  - NADPH
KW  - organoarsenicals
KW  - Paracoccus denitrificans
KW  - redox-responsive repressor
UR  - https://www.mdpi.com/2076-3921/11/5/902
N2  - Paracoccus denitrificans ArsH is encoded by two identical genes located in two distinct putative arsenic resistance (ars) operons. Escherichia coli-produced recombinant N-His6-ArsH was characterized both structurally and kinetically. The X-ray structure of ArsH revealed a flavodoxin-like domain and motifs for the binding of flavin mononucleotide (FMN) and reduced nicotinamide adenine dinucleotide phosphate (NADPH). The protein catalyzed FMN reduction by NADPH via ternary complex mechanism. At a fixed saturating FMN concentration, it acted as an NADPH-dependent organoarsenic reductase displaying ping-pong kinetics. A 1:1 enzymatic reaction of phenylarsonic acid with the reduced form of FMN (FMNH2) and formation of phenylarsonous acid were observed. Growth experiments with P. denitrificans and E. coli revealed increased toxicity of phenylarsonic acid to cells expressing arsH, which may be related to in vivo conversion of pentavalent As to more toxic trivalent form. ArsH expression was upregulated not only by arsenite, but also by redox-active agents paraquat, tert-butyl hydroperoxide and diamide. A crucial role is played by the homodimeric transcriptional repressor ArsR, which was shown in in vitro experiments to monomerize and release from the DNA-target site. Collectively, our results establish ArsH as responsible for enhancement of organo-As(V) toxicity and demonstrate redox control of ars operon.
ER  -

SEDLÁČEK, Vojtěch, Martin KRYL and Igor KUČERA. The ArsH Protein Product of the Paracoccus denitrificans ars Operon Has an Activity of Organoarsenic Reductase and Is Regulated by a Redox-Responsive Repressor. \textit{Antioxidants}. Basel: MDPI, 2022, vol.~11, No~5, p.~1-18. ISSN~2076-3921. Available from: https://dx.doi.org/10.3390/antiox11050902.

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