| PIVATO, Roberto, Šimon KLIMOVIČ, Daniil KABANOV, Filip SVĚRÁK, Martin PEŠL, Jan PŘIBYL a Vladimír ROTREKL. hESC derived cardiomyocyte biosensor to detect the different types of arrhythmogenic properties of drugs. Analytica Chimica Acta. AMSTERDAM: ELSEVIER, 2022, roč. 1216, July 2022, s. 1-10. ISSN 0003-2670. doi:10.1016/j.aca.2022.339959. |
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@article{1860329,
author = {Pivato, Roberto and Klimovič, Šimon and Kabanov, Daniil and Svěrák, Filip and Pešl, Martin and Přibyl, Jan and Rotrekl, Vladimír},
article_location = {AMSTERDAM},
article_number = {July 2022},
doi = {http://dx.doi.org/10.1016/j.aca.2022.339959},
keywords = {Cell-based biosensor; Atomic force microscopy; Human embryonic stem cells; Cardiac arrhythmia; Cardiomyocytes; Caffeine},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {hESC derived cardiomyocyte biosensor to detect the different types of arrhythmogenic properties of drugs},
url = {https://www.sciencedirect.com/science/article/pii/S000326702200530X},
volume = {1216},
year = {2022}
}
TY - JOUR
ID - 1860329
AU - Pivato, Roberto - Klimovič, Šimon - Kabanov, Daniil - Svěrák, Filip - Pešl, Martin - Přibyl, Jan - Rotrekl, Vladimír
PY - 2022
TI - hESC derived cardiomyocyte biosensor to detect the different types of arrhythmogenic properties of drugs
JF - Analytica Chimica Acta
VL - 1216
IS - July 2022
SP - 1-10
EP - 1-10
PB - ELSEVIER
SN - 00032670
KW - Cell-based biosensor
KW - Atomic force microscopy
KW - Human embryonic stem cells
KW - Cardiac arrhythmia
KW - Cardiomyocytes
KW - Caffeine
UR - https://www.sciencedirect.com/science/article/pii/S000326702200530X
N2 - In the present work, we introduce a new cell-based biosensor for detecting arrhythmias based on a novel utilization of the combination of the Atomic Force Microscope (AFM) lateral force measurement as a nanosensor with a dual 3D cardiomyocyte syncytium. Two spontaneously coupled clusters of cardiomyocytes form this. The syncytium's functional contraction behavior was assessed using video sequences analyzed with Musclemotion ImageJ/Fiji software, and immunocytochemistry evaluated phenotype composition. The application of caffeine solution induced arrhythmia as a model drug, and its spontaneous resolution was monitored by AFM lateral force recording and interpretation and calcium fluorescence imaging as a reference method describing non-synchronized contractions of cardiomyocytes. The phenotypic analysis revealed the syncytium as a functional contractile and conduction cardiac behavior model. Calcium fluorescence imaging was used to validate that AFM fully enabled to discriminate cardiac arrhythmias in this in vitro cellular model. The described novel 3D hESCs-based cellular biosensor is suitable to detect arrhythmic events on the level of cardiac contractile and conduction tissue cellular model. The resulting biosensor allows for screening of arrhythmogenic properties of tailored drugs enabling its use in precision medicine.
ER -
PIVATO, Roberto, Šimon KLIMOVIČ, Daniil KABANOV, Filip SVĚRÁK, Martin PEŠL, Jan PŘIBYL a Vladimír ROTREKL. hESC derived cardiomyocyte biosensor to detect the different types of arrhythmogenic properties of drugs. \textit{Analytica Chimica Acta}. AMSTERDAM: ELSEVIER, 2022, roč.~1216, July 2022, s.~1-10. ISSN~0003-2670. doi:10.1016/j.aca.2022.339959.
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