Fatty Acid Supplementation Affects Skin Wound Healing in a Rat
Model

J 2022

Fatty Acid Supplementation Affects Skin Wound Healing in a Rat Model

HOKYNKOVÁ, Alica, Marie NOVÁKOVÁ, Petr BABULA, Miroslava SEDLÁČKOVÁ, Hana PAULOVÁ et. al.

Basic information

Original name

Fatty Acid Supplementation Affects Skin Wound Healing in a Rat Model

Authors

HOKYNKOVÁ, Alica (703 Slovakia, belonging to the institution), Marie NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Petr BABULA (203 Czech Republic, belonging to the institution), Miroslava SEDLÁČKOVÁ (203 Czech Republic, belonging to the institution), Hana PAULOVÁ (203 Czech Republic, belonging to the institution), Miroslava HLAVÁČOVÁ (703 Slovakia, belonging to the institution), Daniela CHARWÁTOVÁ (203 Czech Republic, belonging to the institution) and Tibor STRAČINA (703 Slovakia, guarantor, belonging to the institution)

Edition

Nutrients, Basel, MDPI, 2022, 2072-6643

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30308 Nutrition, Dietetics

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.900

RIV identification code

RIV/00216224:14110/22:00126026

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/nu14112245

UT WoS

000809923600001

Keywords in English

polyunsaturated fatty acids; wound; healing; oxidative stress; 4-hydroxy-2-nonenal

Abstract

V originále

Polyunsaturated fatty acids (PUFA) play an important role in reparative processes. The ratio of PUFAs n-3 to n-6 may affect wound healing. The study aimed to evaluate the effect of dietary supplementation with n-3 and n-6 PUFA in two proportions on skin wounds in laboratory rats. Adult male Wistar rats received 20% fat emulsion with a ratio of 1.4:1 (group A) or 4.3:1 (group B) for n-3:n-6 PUFAs at a daily dose of 1 mL/kg. The control group received water under the same conditions. The animals were supplemented a week before and a week after the skin excision performed on the back. The level of wound closure, various parameters of oxidative stress, and plasma fatty acids composition were evaluated. Wound tissue samples were examined by electron microscopy. The administration of fat emulsions led to significant changes in plasma polyunsaturated fatty acid composition. The increased production of reactive nitrogen species, as well as more numerous newly formed blood vessels and a greater amount of highly organized collagen fibrils in both groups A and B may indicate more intensive healing of the skin wound in rats supplemented with polyunsaturated fatty acids in high n-3:n-6 ratio.

Links

MUNI/A/0910/2017, interní kód MU

Name: Studium molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: stumopo)

Investor: Masaryk University, Category A

MUNI/A/1246/2020, interní kód MU

Name: Kardiovaskulární systém: od iontového kanálu k celotělovému modelu (Acronym: KAVASYKAMO)

Investor: Masaryk University

MUNI/A/1255/2018, interní kód MU

Name: Kardiovaskulární systém a jeho regulace a dysregulace pod vlivem farmak (Acronym: KAREDYSFAR)

Investor: Masaryk University, Category A

MUNI/A/1307/2019, interní kód MU

Name: Kardiovaskulární systém od A do Z (Acronym: KAVASAZ)

Investor: Masaryk University, Category A

Citovat

HOKYNKOVÁ, Alica, Marie NOVÁKOVÁ, Petr BABULA, Miroslava SEDLÁČKOVÁ, Hana PAULOVÁ, Miroslava HLAVÁČOVÁ, Daniela CHARWÁTOVÁ and Tibor STRAČINA. Fatty Acid Supplementation Affects Skin Wound Healing in a Rat Model. Nutrients. Basel: MDPI, 2022, vol. 14, No 11, p. 1-13. ISSN 2072-6643. Available from: https://dx.doi.org/10.3390/nu14112245.

@article{1860557,
   author = {Hokynková, Alica and Nováková, Marie and Babula, Petr and Sedláčková, Miroslava and Paulová, Hana and Hlaváčová, Miroslava and Charwátová, Daniela and Stračina, Tibor},
   article_location = {Basel},
   article_number = {11},
   doi = {http://dx.doi.org/10.3390/nu14112245},
   keywords = {polyunsaturated fatty acids; wound; healing; oxidative stress; 4-hydroxy-2-nonenal},
   language = {eng},
   issn = {2072-6643},
   journal = {Nutrients},
   title = {Fatty Acid Supplementation Affects Skin Wound Healing in a Rat Model},
   url = {https://www.mdpi.com/2072-6643/14/11/2245},
   volume = {14},
   year = {2022}
}

TY  - JOUR
ID  - 1860557
AU  - Hokynková, Alica - Nováková, Marie - Babula, Petr - Sedláčková, Miroslava - Paulová, Hana - Hlaváčová, Miroslava - Charwátová, Daniela - Stračina, Tibor
PY  - 2022
TI  - Fatty Acid Supplementation Affects Skin Wound Healing in a Rat Model
JF  - Nutrients
VL  - 14
IS  - 11
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 20726643
KW  - polyunsaturated fatty acids
KW  - wound
KW  - healing
KW  - oxidative stress
KW  - 4-hydroxy-2-nonenal
UR  - https://www.mdpi.com/2072-6643/14/11/2245
N2  - Polyunsaturated fatty acids (PUFA) play an important role in reparative processes. The ratio of PUFAs n-3 to n-6 may affect wound healing. The study aimed to evaluate the effect of dietary supplementation with n-3 and n-6 PUFA in two proportions on skin wounds in laboratory rats. Adult male Wistar rats received 20% fat emulsion with a ratio of 1.4:1 (group A) or 4.3:1 (group B) for n-3:n-6 PUFAs at a daily dose of 1 mL/kg. The control group received water under the same conditions. The animals were supplemented a week before and a week after the skin excision performed on the back. The level of wound closure, various parameters of oxidative stress, and plasma fatty acids composition were evaluated. Wound tissue samples were examined by electron microscopy. The administration of fat emulsions led to significant changes in plasma polyunsaturated fatty acid composition. The increased production of reactive nitrogen species, as well as more numerous newly formed blood vessels and a greater amount of highly organized collagen fibrils in both groups A and B may indicate more intensive healing of the skin wound in rats supplemented with polyunsaturated fatty acids in high n-3:n-6 ratio.
ER  -

HOKYNKOVÁ, Alica, Marie NOVÁKOVÁ, Petr BABULA, Miroslava SEDLÁČKOVÁ, Hana PAULOVÁ, Miroslava HLAVÁČOVÁ, Daniela CHARWÁTOVÁ and Tibor STRAČINA. Fatty Acid Supplementation Affects Skin Wound Healing in a Rat Model. \textit{Nutrients}. Basel: MDPI, 2022, vol.~14, No~11, p.~1-13. ISSN~2072-6643. Available from: https://dx.doi.org/10.3390/nu14112245.

Detailed Information on Publication Record