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@article{1860588,
author = {Kotasová, Hana and Capandová, Michaela and Pelková, Vendula and Dumková, Jana and Sumbalová Koledová, Zuzana and Remšík, Ján and Souček, Karel and Garlíková, Zuzana and Sedláková, Veronika and Rabata, Anas and Vaňhara, Petr and Moráň, Lukáš and Pečinka, Lukáš and Porokh, Volodymyr and Kučírek, Martin and Streit, Libor and Havel, Josef and Hampl, Aleš},
article_location = {SEOUL},
article_number = {5},
doi = {http://dx.doi.org/10.1007/s13770-022-00458-0},
keywords = {Lung; Epithelium; hESC; Differentiation; Foregut endoderm},
language = {eng},
issn = {1738-2696},
journal = {TISSUE ENGINEERING AND REGENERATIVE MEDICINE},
title = {Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells},
url = {https://link.springer.com/article/10.1007/s13770-022-00458-0},
volume = {19},
year = {2022}
}
TY - JOUR
ID - 1860588
AU - Kotasová, Hana - Capandová, Michaela - Pelková, Vendula - Dumková, Jana - Sumbalová Koledová, Zuzana - Remšík, Ján - Souček, Karel - Garlíková, Zuzana - Sedláková, Veronika - Rabata, Anas - Vaňhara, Petr - Moráň, Lukáš - Pečinka, Lukáš - Porokh, Volodymyr - Kučírek, Martin - Streit, Libor - Havel, Josef - Hampl, Aleš
PY - 2022
TI - Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
JF - TISSUE ENGINEERING AND REGENERATIVE MEDICINE
VL - 19
IS - 5
SP - 1033-1050
EP - 1033-1050
PB - KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
SN - 17382696
KW - Lung
KW - Epithelium
KW - hESC
KW - Differentiation
KW - Foregut endoderm
UR - https://link.springer.com/article/10.1007/s13770-022-00458-0
N2 - Background The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. Methods By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. Results These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. Conclusion Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.
ER -
KOTASOVÁ, Hana, Michaela CAPANDOVÁ, Vendula PELKOVÁ, Jana DUMKOVÁ, Zuzana SUMBALOVÁ KOLEDOVÁ, Ján REMŠÍK, Karel SOUČEK, Zuzana GARLÍKOVÁ, Veronika SEDLÁKOVÁ, Anas RABATA, Petr VAŇHARA, Lukáš MORÁŇ, Lukáš PEČINKA, Volodymyr POROKH, Martin KUČÍREK, Libor STREIT, Josef HAVEL and Aleš HAMPL. Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells. \textit{TISSUE ENGINEERING AND REGENERATIVE MEDICINE}. SEOUL: KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC, 2022, vol.~19, No~5, p.~1033-1050. ISSN~1738-2696. Available from: https://dx.doi.org/10.1007/s13770-022-00458-0.
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