Expandable Lung Epithelium Differentiated from Human Embryonic
Stem Cells

J 2022

Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells

KOTASOVÁ, Hana, Michaela CAPANDOVÁ, Vendula PELKOVÁ, Jana DUMKOVÁ, Zuzana SUMBALOVÁ KOLEDOVÁ et. al.

Basic information

Original name

Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells

Authors

KOTASOVÁ, Hana (203 Czech Republic, belonging to the institution), Michaela CAPANDOVÁ (203 Czech Republic, belonging to the institution), Vendula PELKOVÁ (203 Czech Republic, belonging to the institution), Jana DUMKOVÁ (203 Czech Republic, belonging to the institution), Zuzana SUMBALOVÁ KOLEDOVÁ (703 Slovakia, belonging to the institution), Ján REMŠÍK (703 Slovakia, belonging to the institution), Karel SOUČEK (203 Czech Republic, belonging to the institution), Zuzana GARLÍKOVÁ (203 Czech Republic, belonging to the institution), Veronika SEDLÁKOVÁ (203 Czech Republic, belonging to the institution), Anas RABATA (760 Syrian Arab Republic, belonging to the institution), Petr VAŇHARA (203 Czech Republic, belonging to the institution), Lukáš MORÁŇ (203 Czech Republic, belonging to the institution), Lukáš PEČINKA (203 Czech Republic, belonging to the institution), Volodymyr POROKH (804 Ukraine, belonging to the institution), Martin KUČÍREK (203 Czech Republic, belonging to the institution), Libor STREIT (203 Czech Republic, belonging to the institution), Josef HAVEL (203 Czech Republic, belonging to the institution) and Aleš HAMPL (203 Czech Republic, guarantor, belonging to the institution)

Edition

TISSUE ENGINEERING AND REGENERATIVE MEDICINE, SEOUL, KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC, 2022, 1738-2696

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20602 Medical laboratory technology ;

Country of publisher

Republic of Korea

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.600

RIV identification code

RIV/00216224:14110/22:00126034

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s13770-022-00458-0

UT WoS

000807342200001

Keywords in English

Lung; Epithelium; hESC; Differentiation; Foregut endoderm

Abstract

V originále

Background The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. Methods By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. Results These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. Conclusion Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.

Links

MUNI/A/1390/2020, interní kód MU

Name: Analytická a fyzikální chemie ve výzkumu biologických, geologických a syntetických materiálů (Acronym: BIOGEOSYNT)

Investor: Masaryk University

MUNI/A/1398/2021, interní kód MU

Name: Zdroje pro tkáňové inženýrství 12 (Acronym: TissueEng 12)

Investor: Masaryk University

MUNI/A/1689/2020, interní kód MU

Name: Zdroje pro tkáňové inženýrství 11 (Acronym: TissueEng 11)

Investor: Masaryk University

NV16-31501A, research and development project

Name: Tkáňové inženýrství epitelů: Buňky a protokoly pro regenerativní medicínu

ROZV/28/LF23/2020, interní kód MU

Name: Objasněni vlivu ztráty TUSC3 na změnu fenotypových vlastnosti buněk ovariálního povrchového epitelu.

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

KOTASOVÁ, Hana, Michaela CAPANDOVÁ, Vendula PELKOVÁ, Jana DUMKOVÁ, Zuzana SUMBALOVÁ KOLEDOVÁ, Ján REMŠÍK, Karel SOUČEK, Zuzana GARLÍKOVÁ, Veronika SEDLÁKOVÁ, Anas RABATA, Petr VAŇHARA, Lukáš MORÁŇ, Lukáš PEČINKA, Volodymyr POROKH, Martin KUČÍREK, Libor STREIT, Josef HAVEL and Aleš HAMPL. Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells. TISSUE ENGINEERING AND REGENERATIVE MEDICINE. SEOUL: KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC, 2022, vol. 19, No 5, p. 1033-1050. ISSN 1738-2696. Available from: https://dx.doi.org/10.1007/s13770-022-00458-0.

@article{1860588,
   author = {Kotasová, Hana and Capandová, Michaela and Pelková, Vendula and Dumková, Jana and Sumbalová Koledová, Zuzana and Remšík, Ján and Souček, Karel and Garlíková, Zuzana and Sedláková, Veronika and Rabata, Anas and Vaňhara, Petr and Moráň, Lukáš and Pečinka, Lukáš and Porokh, Volodymyr and Kučírek, Martin and Streit, Libor and Havel, Josef and Hampl, Aleš},
   article_location = {SEOUL},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s13770-022-00458-0},
   keywords = {Lung; Epithelium; hESC; Differentiation; Foregut endoderm},
   language = {eng},
   issn = {1738-2696},
   journal = {TISSUE ENGINEERING AND REGENERATIVE MEDICINE},
   title = {Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells},
   url = {https://link.springer.com/article/10.1007/s13770-022-00458-0},
   volume = {19},
   year = {2022}
}

TY  - JOUR
ID  - 1860588
AU  - Kotasová, Hana - Capandová, Michaela - Pelková, Vendula - Dumková, Jana - Sumbalová Koledová, Zuzana - Remšík, Ján - Souček, Karel - Garlíková, Zuzana - Sedláková, Veronika - Rabata, Anas - Vaňhara, Petr - Moráň, Lukáš - Pečinka, Lukáš - Porokh, Volodymyr - Kučírek, Martin - Streit, Libor - Havel, Josef - Hampl, Aleš
PY  - 2022
TI  - Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
JF  - TISSUE ENGINEERING AND REGENERATIVE MEDICINE
VL  - 19
IS  - 5
SP  - 1033-1050
EP  - 1033-1050
PB  - KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
SN  - 17382696
KW  - Lung
KW  - Epithelium
KW  - hESC
KW  - Differentiation
KW  - Foregut endoderm
UR  - https://link.springer.com/article/10.1007/s13770-022-00458-0
N2  - Background The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. Methods By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. Results These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. Conclusion Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.
ER  -

KOTASOVÁ, Hana, Michaela CAPANDOVÁ, Vendula PELKOVÁ, Jana DUMKOVÁ, Zuzana SUMBALOVÁ KOLEDOVÁ, Ján REMŠÍK, Karel SOUČEK, Zuzana GARLÍKOVÁ, Veronika SEDLÁKOVÁ, Anas RABATA, Petr VAŇHARA, Lukáš MORÁŇ, Lukáš PEČINKA, Volodymyr POROKH, Martin KUČÍREK, Libor STREIT, Josef HAVEL and Aleš HAMPL. Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells. \textit{TISSUE ENGINEERING AND REGENERATIVE MEDICINE}. SEOUL: KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC, 2022, vol.~19, No~5, p.~1033-1050. ISSN~1738-2696. Available from: https://dx.doi.org/10.1007/s13770-022-00458-0.

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