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@article{1862251,
author = {Folta, Adam and Sasinkova, Markéta and Ďuriníková, Anna and Drncová, Marie and Weinbergerová, Barbora and Mayer, Jiří and Ježíšková, Ivana},
article_location = {DORDRECHT},
article_number = {8},
doi = {http://dx.doi.org/10.1007/s11033-022-07363-8},
keywords = {Nucleophosmine 1; Acute myeloid leukemia; Quantitative PCR standards; Colony selection; Cloning},
language = {eng},
issn = {0301-4851},
journal = {Molecular Biology Reports},
title = {Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia.},
url = {https://link.springer.com/article/10.1007/s11033-022-07363-8},
volume = {49},
year = {2022}
}
TY - JOUR
ID - 1862251
AU - Folta, Adam - Sasinkova, Markéta - Ďuriníková, Anna - Drncová, Marie - Weinbergerová, Barbora - Mayer, Jiří - Ježíšková, Ivana
PY - 2022
TI - Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia.
JF - Molecular Biology Reports
VL - 49
IS - 8
SP - 8169-8172
EP - 8169-8172
PB - SPRINGER
SN - 03014851
KW - Nucleophosmine 1
KW - Acute myeloid leukemia
KW - Quantitative PCR standards
KW - Colony selection
KW - Cloning
UR - https://link.springer.com/article/10.1007/s11033-022-07363-8
N2 - Background NPM1 plasmid standards are required for absolute quantification of minimal residual disease in acute myeloid leukemia patients. The standards are usually obtained, next to commercially constructed gene fragments, from transgenic bacteria colonies. However, this procedure is laborious and very time consuming. Methods and results We have developed a PCR method that speeds up, simplifies, and streamlines the process of preparing NPM1 plasmid standards. The method is based on a combination of three primers, two surrounding the usual NPM1 mutation position and one over the mutation site. With this method, we were able to clearly distinguish plasmids with at least 15 different NPM1 mutations from the wild-type NPM1 plasmid. Conclusions With the new approach, preparing NPM1 plasmid standards is easier, identifying NPM1-positive colonies is possible in less than a day and moreover, for a lower price than commercially constructed gene fragments.
ER -
FOLTA, Adam, Markéta SASINKOVA, Anna ĎURINÍKOVÁ, Marie DRNCOVÁ, Barbora WEINBERGEROVÁ, Jiří MAYER and Ivana JEŽÍŠKOVÁ. Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia. \textit{Molecular Biology Reports}. DORDRECHT: SPRINGER, 2022, vol.~49, No~8, p.~8169-8172. ISSN~0301-4851. Available from: https://dx.doi.org/10.1007/s11033-022-07363-8.
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