Colloidal lithography as a novel approach for the development
of Ni-nanocavity insulin sensor

J 2022

Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor

ŠIŠOLÁKOVÁ, Ivana, Ondrej PETRUŠ, Jana SHEPA, Zdeněk FARKA, Andrej ORIŇÁK et. al.

Basic information

Original name

Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor

Authors

ŠIŠOLÁKOVÁ, Ivana (703 Slovakia), Ondrej PETRUŠ (703 Slovakia), Jana SHEPA (703 Slovakia), Zdeněk FARKA (203 Czech Republic, guarantor, belonging to the institution), Andrej ORIŇÁK (703 Slovakia) and Renáta ORIŇAKOVÁ

Edition

Scientific Reports, Springer Nature, 2022, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

21001 Nano-materials

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600

RIV identification code

RIV/00216224:14310/22:00129981

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41598-022-15283-7

UT WoS

001014944000030

Keywords in English

electrochemical sensor; insulin; colloidal lithography; nanostructures

Abstract

V originále

In this study, a highly sensitive, fast, and selective enzyme-free electrochemical sensor based on the deposition of Ni cavities on conductive glass was proposed for insulin detection. Considering the growing prevalence of diabetes mellitus, an electrochemical sensor for the determination of insulin was proposed for the effective diagnosis of the disease. Colloidal lithography enabled deposition of nanostructured layer (substrate) with homogeneous distribution of Ni cavities on the electrode surface with a large active surface area. The morphology and structure of conductive indium tin oxide glass modified with Ni cavities (Ni-c-ITO) were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The diameter of the resulting cavities was approximately 500 nm, while their depth was calculated at 190 ± 4 nm and 188 ± 18 nm using AFM and SEM, respectively. The insulin assay performance was evaluated by cyclic voltammetry. Ni-c-ITO exhibited excellent analytical characteristics, including high sensitivity (1.032 µA µmol−1 dm3), a low detection limit (156 µmol dm−3), and a wide dynamic range (500 nmol dm−3 to 10 µmol dm−3). Finally, the determination of insulin in buffer with interferents and in real blood serum samples revealed high specificity and demonstrated the practical potential of the method.

Links

22020140, interní kód MU

Name: Věda v zemích V4 - výzkum nových senzorů pro diagnostiku diabetu (Acronym: Věda v zemích V4 - výzkum nových senzorů)

Investor: International Visegrad Fund (IVF)

90127, large research infrastructures

Name: CIISB II

Citovat

ŠIŠOLÁKOVÁ, Ivana, Ondrej PETRUŠ, Jana SHEPA, Zdeněk FARKA, Andrej ORIŇÁK and Renáta ORIŇAKOVÁ. Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor. Scientific Reports. Springer Nature, 2022, vol. 12, No 1, p. 1-12. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-022-15283-7.

@article{1863572,
   author = {Šišoláková, Ivana and Petruš, Ondrej and Shepa, Jana and Farka, Zdeněk and Oriňák, Andrej and Oriňaková, Renáta},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-022-15283-7},
   keywords = {electrochemical sensor; insulin; colloidal lithography; nanostructures},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor},
   url = {https://www.nature.com/articles/s41598-022-15283-7},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 1863572
AU  - Šišoláková, Ivana - Petruš, Ondrej - Shepa, Jana - Farka, Zdeněk - Oriňák, Andrej - Oriňaková, Renáta
PY  - 2022
TI  - Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor
JF  - Scientific Reports
VL  - 12
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - Springer Nature
SN  - 20452322
KW  - electrochemical sensor
KW  - insulin
KW  - colloidal lithography
KW  - nanostructures
UR  - https://www.nature.com/articles/s41598-022-15283-7
N2  - In this study, a highly sensitive, fast, and selective enzyme-free electrochemical sensor based on the deposition of Ni cavities on conductive glass was proposed for insulin detection. Considering the growing prevalence of diabetes mellitus, an electrochemical sensor for the determination of insulin was proposed for the effective diagnosis of the disease. Colloidal lithography enabled deposition of nanostructured layer (substrate) with homogeneous distribution of Ni cavities on the electrode surface with a large active surface area. The morphology and structure of conductive indium tin oxide glass modified with Ni cavities (Ni-c-ITO) were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The diameter of the resulting cavities was approximately 500 nm, while their depth was calculated at 190 ± 4 nm and 188 ± 18 nm using AFM and SEM, respectively. The insulin assay performance was evaluated by cyclic voltammetry. Ni-c-ITO exhibited excellent analytical characteristics, including high sensitivity (1.032 µA µmol−1 dm3), a low detection limit (156 µmol dm−3), and a wide dynamic range (500 nmol dm−3 to 10 µmol dm−3). Finally, the determination of insulin in buffer with interferents and in real blood serum samples revealed high specificity and demonstrated the practical potential of the method.
ER  -

ŠIŠOLÁKOVÁ, Ivana, Ondrej PETRUŠ, Jana SHEPA, Zdeněk FARKA, Andrej ORIŇÁK and Renáta ORIŇAKOVÁ. Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor. \textit{Scientific Reports}. Springer Nature, 2022, vol.~12, No~1, p.~1-12. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-022-15283-7.

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