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@article{1863572,
author = {Šišoláková, Ivana and Petruš, Ondrej and Shepa, Jana and Farka, Zdeněk and Oriňák, Andrej and Oriňaková, Renáta},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-022-15283-7},
keywords = {electrochemical sensor; insulin; colloidal lithography; nanostructures},
language = {eng},
issn = {2045-2322},
journal = {Scientific Reports},
title = {Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor},
url = {https://www.nature.com/articles/s41598-022-15283-7},
volume = {12},
year = {2022}
}
TY - JOUR
ID - 1863572
AU - Šišoláková, Ivana - Petruš, Ondrej - Shepa, Jana - Farka, Zdeněk - Oriňák, Andrej - Oriňaková, Renáta
PY - 2022
TI - Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor
JF - Scientific Reports
VL - 12
IS - 1
SP - 1-12
EP - 1-12
PB - Springer Nature
SN - 20452322
KW - electrochemical sensor
KW - insulin
KW - colloidal lithography
KW - nanostructures
UR - https://www.nature.com/articles/s41598-022-15283-7
N2 - In this study, a highly sensitive, fast, and selective enzyme-free electrochemical sensor based on the deposition of Ni cavities on conductive glass was proposed for insulin detection. Considering the growing prevalence of diabetes mellitus, an electrochemical sensor for the determination of insulin was proposed for the effective diagnosis of the disease. Colloidal lithography enabled deposition of nanostructured layer (substrate) with homogeneous distribution of Ni cavities on the electrode surface with a large active surface area. The morphology and structure of conductive indium tin oxide glass modified with Ni cavities (Ni-c-ITO) were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The diameter of the resulting cavities was approximately 500 nm, while their depth was calculated at 190 ± 4 nm and 188 ± 18 nm using AFM and SEM, respectively. The insulin assay performance was evaluated by cyclic voltammetry. Ni-c-ITO exhibited excellent analytical characteristics, including high sensitivity (1.032 µA µmol−1 dm3), a low detection limit (156 µmol dm−3), and a wide dynamic range (500 nmol dm−3 to 10 µmol dm−3). Finally, the determination of insulin in buffer with interferents and in real blood serum samples revealed high specificity and demonstrated the practical potential of the method.
ER -
ŠIŠOLÁKOVÁ, Ivana, Ondrej PETRUŠ, Jana SHEPA, Zdeněk FARKA, Andrej ORIŇÁK and Renáta ORIŇAKOVÁ. Colloidal lithography as a novel approach for the development of Ni-nanocavity insulin sensor. \textit{Scientific Reports}. Springer Nature, 2022, vol.~12, No~1, p.~1-12. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-022-15283-7.
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