This article focuses on a so-called micro-expression analysis and its applicability in a criminal procedure. At first, it presents foundations and development of this method and its current state of art, including the difference information technologies have made thereto. Then it proceeds to a comparison of the said method with a physio-detection examination (i.e. common lie detection method with the use of a lie detector) in the terms of their natures, legal limitations for the evidentiary value of their outcomes including their reasons. It finishes with an own analysis of applicability of the method of micro-expression analysis in criminal proceedings and conditions upon which the conclusion lies.