J 2022

In vitro testicular toxicity of environmentally relevant endocrine-disrupting chemicals: 2D vs. 3D models of prepubertal Leydig TM3 cells

SYCHROVÁ, Eliška, Affiefa YAWER, Petra LABOHÁ, Amrita BASU, Aneta DYDOWICZOVÁ et. al.

Basic information

Original name

In vitro testicular toxicity of environmentally relevant endocrine-disrupting chemicals: 2D vs. 3D models of prepubertal Leydig TM3 cells

Authors

SYCHROVÁ, Eliška (203 Czech Republic, belonging to the institution), Affiefa YAWER (586 Pakistan, belonging to the institution), Petra LABOHÁ (203 Czech Republic, belonging to the institution), Amrita BASU (356 India, belonging to the institution), Aneta DYDOWICZOVÁ (203 Czech Republic, belonging to the institution), Ishita VIRMANI (356 India, belonging to the institution), Pavel BABICA (203 Czech Republic, belonging to the institution) and Iva SOVADINOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Environmental Toxicology and Pharmacology, Amsterdam, Elsevier Science B.V. 2022, 1382-6689

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.300

RIV identification code

RIV/00216224:14310/22:00126192

Organization unit

Faculty of Science

DOI

UT WoS

000806756800002

Keywords in English

3D in vitro models; Endocrine-disrupting chemicals; Leydig cells; Lipotoxicity; Reproductive toxicity; Spheroids

Tags

Tags

International impact, Reviewed
Změněno: 3/4/2023 09:30, Mgr. Marie Šípková, DiS.

Abstract

V originále

The testis is a priority organ for developing alternative models to assess male reproductive health hazards of chemicals. This study characterized a 3D in vitro model of murine prepubertal Leydig TM3 cells with improved expression of steroidogenesis markers suitable for image-based screening of testicular toxicity. This 3D scaffold free spheroid model was applied to explore the impact of prototypical endocrine-disrupting chemicals (EDCs) and environmental reprotoxicants (benzo[a]pyrene, 2-and 9-methylanthracenes, fluoranthene, triclosan, triclocarban, methoxychlor) on male reproductive health. The results were compared to the male reprotoxicity potential of EDCs assessed in a traditional monolayer (2D) culture. The testicular toxicity was dependent not only on the type of culture (2D vs. 3D models) but also on the duration of exposure. Benzo[a]pyrene and triclocarban were the most active compounds, eliciting cytotoxic effects in prepubertal Leydig cells at low micromolar concentrations, which might be a mechanism contributing to their male reprotoxicity.

Links

EF17_043/0009632, research and development project
Name: CETOCOEN Excellence
EF19_073/0016943, research and development project
Name: Interní grantová agentura Masarykovy univerzity
GA22-30004S, research and development project
Name: Alternativní přístupy v hodnocení mužské reprodukční toxicity chemických směsí bez využití zvířat
Investor: Czech Science Foundation
GJ16-10775Y, research and development project
Name: Mezibuněčná komunikace zprostředkovaná mezerovými spoji jako cíl endokrinních disruptorů v testikulárních buňkách
Investor: Czech Science Foundation
LM2018121, research and development project
Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI
857560, interní kód MU
(CEP code: EF17_043/0009632)
Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)
Investor: European Union, Spreading excellence and widening participation