Nanodiamonds as traps for fibroblast growth factors: Parameters
influencing the interaction

J 2022

Nanodiamonds as traps for fibroblast growth factors: Parameters influencing the interaction

MIKESOVA, Jana, Daria MILIAIEVA, Pavla STENCLOVA, Marek KINDERMANN, Tereza VUCKOVA et. al.

Basic information

Original name

Nanodiamonds as traps for fibroblast growth factors: Parameters influencing the interaction

Authors

MIKESOVA, Jana (203 Czech Republic), Daria MILIAIEVA, Pavla STENCLOVA (203 Czech Republic), Marek KINDERMANN (203 Czech Republic), Tereza VUCKOVA (203 Czech Republic), Marcela MADLIKOVA (203 Czech Republic), Milan FABRY (203 Czech Republic), Vaclav VEVERKA (203 Czech Republic), Jiri SCHIMER (203 Czech Republic), Pavel KREJČÍ (203 Czech Republic, belonging to the institution), Stepan STEHLIK (203 Czech Republic) and Petr CIGLER (203 Czech Republic, guarantor)

Edition

Carbon, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2022, 0008-6223

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 10.900

RIV identification code

RIV/00216224:14110/22:00126271

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.carbon.2022.04.017

UT WoS

000831026300009

Keywords in English

Nanodiamond; Fibroblast growth factor; Binding; Sequestration; Thermodynamics

Abstract

V originále

Fibroblast growth factors (FGFs) deliver external cell communication signals, to regulate mammalian development, metabolism and homeostasis. Blocking the FGF interactions with their receptors at the cell membrane is one of the promising strategies for treatment of diseases related to dysregulated FGF signaling, such as cancer, metabolic syndromes, and developmental disorders. Recently, detonation nanodiamonds (NDs) with positive ζ-potential were identified as highly effective and selective FGF binders, sequestering FGF molecules and preventing their interaction with receptors under physiological conditions, thus potentially eliminating the effect of FGF overexpression. Here, we investigated the influence of ND origin (detonation vs. high-pressure high-temperature), surface modification, size and separation/purification steps on the sequestration ability and binding affinity of FGF2, a representative member of the FGF family. We measured FTIR and Raman spectra of the NDs, assessed their colloidal behavior and ζ-potential, and correlated their properties with FGF2 interaction levels. Using Western blot and ELISA, we quantified the strength of the interaction between detonation NDs and FGF2. All NDs with positive ζ-potential sequestered FGF2 at its physiologically relevant concentrations. Hydrogenated detonation NDs showed the highest binding capacity. Using Langmuir model, we estimated the apparent dissociation constant between FGF2 and detonation ND with positive ζ-potential to be in the nanomolar range in full fetal bovine serum. Because such tight interaction between a protein and a solid nanoparticle occurred in ∼105-fold molar excess of serum proteins, we believe that NDs can potentially be used in vivo as selective FGF traps to regulate disorders caused by aberrant FGF signaling.

Links

90110, large research infrastructures

Name: CzechNanoLab

Citovat

MIKESOVA, Jana, Daria MILIAIEVA, Pavla STENCLOVA, Marek KINDERMANN, Tereza VUCKOVA, Marcela MADLIKOVA, Milan FABRY, Vaclav VEVERKA, Jiri SCHIMER, Pavel KREJČÍ, Stepan STEHLIK and Petr CIGLER. Nanodiamonds as traps for fibroblast growth factors: Parameters influencing the interaction. Carbon. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2022, vol. 195, No 15, p. 372-386. ISSN 0008-6223. Available from: https://dx.doi.org/10.1016/j.carbon.2022.04.017.

@article{1875148,
   author = {Mikesova, Jana and Miliaieva, Daria and Stenclova, Pavla and Kindermann, Marek and Vuckova, Tereza and Madlikova, Marcela and Fabry, Milan and Veverka, Vaclav and Schimer, Jiri and Krejčí, Pavel and Stehlik, Stepan and Cigler, Petr},
   article_location = {OXFORD},
   article_number = {15},
   doi = {http://dx.doi.org/10.1016/j.carbon.2022.04.017},
   keywords = {Nanodiamond; Fibroblast growth factor; Binding; Sequestration; Thermodynamics},
   language = {eng},
   issn = {0008-6223},
   journal = {Carbon},
   title = {Nanodiamonds as traps for fibroblast growth factors: Parameters influencing the interaction},
   url = {https://www.sciencedirect.com/science/article/pii/S0008622322002871},
   volume = {195},
   year = {2022}
}

TY  - JOUR
ID  - 1875148
AU  - Mikesova, Jana - Miliaieva, Daria - Stenclova, Pavla - Kindermann, Marek - Vuckova, Tereza - Madlikova, Marcela - Fabry, Milan - Veverka, Vaclav - Schimer, Jiri - Krejčí, Pavel - Stehlik, Stepan - Cigler, Petr
PY  - 2022
TI  - Nanodiamonds as traps for fibroblast growth factors: Parameters influencing the interaction
JF  - Carbon
VL  - 195
IS  - 15
SP  - 372-386
EP  - 372-386
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00086223
KW  - Nanodiamond
KW  - Fibroblast growth factor
KW  - Binding
KW  - Sequestration
KW  - Thermodynamics
UR  - https://www.sciencedirect.com/science/article/pii/S0008622322002871
N2  - Fibroblast growth factors (FGFs) deliver external cell communication signals, to regulate mammalian development, metabolism and homeostasis. Blocking the FGF interactions with their receptors at the cell membrane is one of the promising strategies for treatment of diseases related to dysregulated FGF signaling, such as cancer, metabolic syndromes, and developmental disorders. Recently, detonation nanodiamonds (NDs) with positive ζ-potential were identified as highly effective and selective FGF binders, sequestering FGF molecules and preventing their interaction with receptors under physiological conditions, thus potentially eliminating the effect of FGF overexpression. Here, we investigated the influence of ND origin (detonation vs. high-pressure high-temperature), surface modification, size and separation/purification steps on the sequestration ability and binding affinity of FGF2, a representative member of the FGF family. We measured FTIR and Raman spectra of the NDs, assessed their colloidal behavior and ζ-potential, and correlated their properties with FGF2 interaction levels. Using Western blot and ELISA, we quantified the strength of the interaction between detonation NDs and FGF2. All NDs with positive ζ-potential sequestered FGF2 at its physiologically relevant concentrations. Hydrogenated detonation NDs showed the highest binding capacity. Using Langmuir model, we estimated the apparent dissociation constant between FGF2 and detonation ND with positive ζ-potential to be in the nanomolar range in full fetal bovine serum. Because such tight interaction between a protein and a solid nanoparticle occurred in ∼105-fold molar excess of serum proteins, we believe that NDs can potentially be used in vivo as selective FGF traps to regulate disorders caused by aberrant FGF signaling.
ER  -

MIKESOVA, Jana, Daria MILIAIEVA, Pavla STENCLOVA, Marek KINDERMANN, Tereza VUCKOVA, Marcela MADLIKOVA, Milan FABRY, Vaclav VEVERKA, Jiri SCHIMER, Pavel KREJČÍ, Stepan STEHLIK and Petr CIGLER. Nanodiamonds as traps for fibroblast growth factors: Parameters influencing the interaction. \textit{Carbon}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2022, vol.~195, No~15, p.~372-386. ISSN~0008-6223. Available from: https://dx.doi.org/10.1016/j.carbon.2022.04.017.

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