SALÁT, Jiří, Petra STRAKOVÁ and Daniel RŮŽEK. Dynamics of Whole Virus and Non-Structural Protein 1 (NS1) IgG Response in Mice Immunized with Two Commercial Tick-Borne Encephalitis Vaccines. Vaccines. MDPI, 2022, vol. 10, No 7, p. 1-6. ISSN 2076-393X. Available from: https://dx.doi.org/10.3390/vaccines10071001.
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Basic information
Original name Dynamics of Whole Virus and Non-Structural Protein 1 (NS1) IgG Response in Mice Immunized with Two Commercial Tick-Borne Encephalitis Vaccines.
Authors SALÁT, Jiří (203 Czech Republic), Petra STRAKOVÁ (203 Czech Republic) and Daniel RŮŽEK (203 Czech Republic, guarantor, belonging to the institution).
Edition Vaccines, MDPI, 2022, 2076-393X.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10607 Virology
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 7.800
RIV identification code RIV/00216224:14310/22:00126325
Organization unit Faculty of Science
Doi http://dx.doi.org/10.3390/vaccines10071001
UT WoS 000833195100001
Keywords in English tick-borne encephalitis virus; vaccine; non-structural protein 1
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 22/11/2022 14:53.
Abstract
The presence of a non-structural protein 1 (NS1) in tick-borne encephalitis (TBE) vaccines and the possible induction of an NS1-specific immune response in vaccinated individuals remains a somewhat controversial topic. Previously, we detected the presence of NS1 in the Encepur TBE vaccine by mass spectrometry and found the induction of NS1-specific IgG antibodies in mice vaccinated with the FSME-Immun TBE vaccine. Here, in this follow-up study, we examined the dynamics and extent of the NS1-specific IgG response in mice vaccinated with these two vaccines in more detail and compared it with the IgG response to the whole virus (WV). Mice were vaccinated at two-week intervals with a total of six doses of each vaccine, and levels of IgG antibodies to TBE virus WV and NS1 were measured by ELISA after each dose. Both vaccines elicited a robust anti-WV IgG response after two doses. The Encepur vaccine did not elicit NS1-specific IgG even after all six doses. In contrast, the FSME-Immun vaccine triggered the production of NS1-specific IgG after four doses. The results indicate that FSME-Immun is the only vaccine that elicits an NS1-specific antibody response in mice. However, compared to WV-specific IgG, the NS1-specific response is weaker, and a higher number of doses is required to induce detectable levels of NS1-specific IgG antibodies.
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