IFITM PROTEINS REGULATE SENSITIVITY OF CERVICAL CANCER CELLS TO
CISPLATIN

a 2022

IFITM PROTEINS REGULATE SENSITIVITY OF CERVICAL CANCER CELLS TO CISPLATIN

FRIEDLOVÁ, Nela, Michaela GALIOVÁ VAŠINOVÁ, Kristýna BILAVČÍKOVÁ, Pavel COUFALÍK, Lenka HERNYCHOVÁ et. al.

Basic information

Original name

IFITM PROTEINS REGULATE SENSITIVITY OF CERVICAL CANCER CELLS TO CISPLATIN

Authors

FRIEDLOVÁ, Nela, Michaela GALIOVÁ VAŠINOVÁ, Kristýna BILAVČÍKOVÁ, Pavel COUFALÍK, Lenka HERNYCHOVÁ, Vlastimil TICHÝ, Ted HUPP, Bořivoj VOJTĚŠEK and Marta NEKULOVÁ

Edition

XXI. Interdisciplinary meeting of young life scientists, 2022

Other information

Type of outcome

Konferenční abstrakt

Confidentiality degree

není předmětem státního či obchodního tajemství

ISSN

Změněno: 5/8/2022 15:03, Mgr. Nela Friedlová

Abstract

V originále

Tumor therapy resistance is a frequent limiting factor to achieve cure of cancer patients. Therefore, an effort to elucidate mechanisms of this phenomenon is essential to make cancer treatment more effective. Upregulation of interferon-related DNA damage resistance signature (IRDS) genes was described in different radio- and chemotherapy resistant tumors. IRDS genes ensure prolonged viral and DNA damage resistance bringing tumors a selective advantage1. Interferon-induced transmembrane proteins (IFITMs), as a part of IRDS, provide cellular defense against broad range of viruses, while contributing to tumor progression. During our investigation focused on IFITM protein functions in tumors we described a linkage between these proteins and cellular response to chemotherapeutic drugs. Using cervical cancer cells, we proved enhanced cisplatin sensitivity of single IFITM1 or IFITM3 knock-out (KO) derivatives. Interestingly, double IFITM1/3 KO clones exert exclusive resistance to cisplatin. Therefore, we further investigate causes of the observed phenomenon. Using inductively coupled plasma mass spectrometry (ICP-MS) technique, we analyzed the cisplatin uptake and efflux rates revealing limited cisplatin influx by IFITM1/3 KO cells. Based on the differentially expressed proteins in IFITM1/3 KO cells after cisplatin treatment identified by pulse SILAC approach we focused our research also on the process of autophagy and vesicle trafficking. We continue to investigate the exact mechanism of IFITM proteins involvement in the chemoresistance of cancer cells.

Citovat

FRIEDLOVÁ, Nela, Michaela GALIOVÁ VAŠINOVÁ, Kristýna BILAVČÍKOVÁ, Pavel COUFALÍK, Lenka HERNYCHOVÁ, Vlastimil TICHÝ, Ted HUPP, Bořivoj VOJTĚŠEK and Marta NEKULOVÁ. IFITM PROTEINS REGULATE SENSITIVITY OF CERVICAL CANCER CELLS TO CISPLATIN. In XXI. Interdisciplinary meeting of young life scientists. 2022. ISSN 2336-7202.

@proceedings{1883508,
   author = {Friedlová, Nela and Galiová Vašinová, Michaela and Bilavčíková, Kristýna and Coufalík, Pavel and Hernychová, Lenka and Tichý, Vlastimil and HUPP, Ted and Vojtěšek, Bořivoj and Nekulová, Marta},
   booktitle = {XXI. Interdisciplinary meeting of young life scientists},
   title = {IFITM PROTEINS REGULATE SENSITIVITY OF CERVICAL CANCER CELLS TO CISPLATIN},
   year = {2022}
}

TY  - CONF
ID  - 1883508
AU  - Friedlová, Nela - Galiová Vašinová, Michaela - Bilavčíková, Kristýna - Coufalík, Pavel - Hernychová, Lenka - Tichý, Vlastimil - HUPP, Ted - Vojtěšek, Bořivoj - Nekulová, Marta
PY  - 2022
TI  - IFITM PROTEINS REGULATE SENSITIVITY OF CERVICAL CANCER CELLS TO CISPLATIN
N2  - Tumor therapy resistance is a frequent limiting factor to achieve cure of cancer patients. Therefore, an effort to elucidate mechanisms of this phenomenon is essential to make cancer treatment more effective. Upregulation of interferon-related DNA damage resistance signature (IRDS) genes was described in different radio- and chemotherapy resistant tumors. IRDS genes ensure prolonged viral and DNA damage resistance bringing tumors a selective advantage1. Interferon-induced transmembrane proteins (IFITMs), as a part of IRDS, provide cellular defense against broad range of viruses, while contributing to tumor progression. During our investigation focused on IFITM protein functions in tumors we described a linkage between these proteins and cellular response to chemotherapeutic drugs. Using cervical cancer cells, we proved enhanced cisplatin sensitivity of single IFITM1 or IFITM3 knock-out (KO) derivatives. Interestingly, double IFITM1/3 KO clones exert exclusive resistance to cisplatin. Therefore, we further investigate causes of the observed phenomenon. Using inductively coupled plasma mass spectrometry (ICP-MS) technique, we analyzed the cisplatin uptake and efflux rates revealing limited cisplatin influx by IFITM1/3 KO cells. Based on the differentially expressed proteins in IFITM1/3 KO cells after cisplatin treatment identified by pulse SILAC approach we focused our research also on the process of autophagy and vesicle trafficking. We continue to investigate the exact mechanism of IFITM proteins involvement in the chemoresistance of cancer cells.
ER  -

FRIEDLOVÁ, Nela, Michaela GALIOVÁ VAŠINOVÁ, Kristýna BILAVČÍKOVÁ, Pavel COUFALÍK, Lenka HERNYCHOVÁ, Vlastimil TICHÝ, Ted HUPP, Bořivoj VOJTĚŠEK and Marta NEKULOVÁ. IFITM PROTEINS REGULATE SENSITIVITY OF CERVICAL CANCER CELLS TO CISPLATIN. In \textit{XXI. Interdisciplinary meeting of young life scientists}. 2022. ISSN~2336-7202.

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