Sex-dependent host-microbiome dynamics in zebrafish:
Implications for toxicology and gastrointestinal physiology

J 2022

Sex-dependent host-microbiome dynamics in zebrafish: Implications for toxicology and gastrointestinal physiology

MARTYNIUK, Christopher J., Amanda N. BUERGER, Hana VESPALCOVÁ, Barbora RUDZANOVÁ, Shahadur R. SOHAG et. al.

Basic information

Original name

Sex-dependent host-microbiome dynamics in zebrafish: Implications for toxicology and gastrointestinal physiology

Authors

MARTYNIUK, Christopher J., Amanda N. BUERGER, Hana VESPALCOVÁ (203 Czech Republic, belonging to the institution), Barbora RUDZANOVÁ (203 Czech Republic, belonging to the institution), Shahadur R. SOHAG, Amy T. HANLON, Pamela E. GINN, Serena L. CRAFT, Soňa SMETANOVÁ (203 Czech Republic, belonging to the institution), Eva BUDINSKÁ (703 Slovakia, belonging to the institution), Joseph H. Jr. BISESI and Ondřej ADAMOVSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, NEW YORK, ELSEVIER SCIENCE INC, 2022, 1744-117X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.000

RIV identification code

RIV/00216224:14310/22:00126366

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.cbd.2022.100993

UT WoS

000798284800002

Keywords in English

Sex differences; Experimental design; Gene networks; Gastrointestinal system; Microgenderome

Abstract

V originále

The physiology of males and females can be vastly different, complicating interpretation of toxicological and physiological data. The objectives of this study were to elucidate the sex differences in the microbiomegastrointestinal (GI) transcriptome of adult zebrafish. We compared microbial composition and diversity in both males and females fed the same diet and housed in the same environment. There were no sex-specific differences in weight gain nor gastrointestinal morphology based on histopathology. There was no difference in gut microbial diversity, richness (Shannon and Chao1 index) nor predicted functional composition of the microbiome between males and females. Prior to post-hoc correction, male zebrafish showed higher abundance for the bacterial families Erythrobacteraceae and Lamiaceae, both belonging to the phyla Actinobacteria and Proteobacteria. At the genus level, Lamia and Altererythrobacter were more dominant in males and an unidentified genus in Bacteroidetes was more abundant in females. There were 16 unique differentially expressed transcripts in the gastrointestinal tissue between male and female zebrafish (FDR corrected, p < 0.05). Relative to males, the mRNA expression for trim35-9, slc25a48, chchd3b, csad, and hsd17b3 were lower in female GI while cyp2k6, adra2c, and bckdk were higher in the female GI. Immune and lipid-related gene network expression differed between the sexes (i.e., cholesterol export and metabolism) as well as networks related to gastric motility, gastrointestinal system absorption and digestion. Such data provide clues as to putative differences in gastrointestinal physiology between male and female zebrafish. This study identifies host-transcriptome differences that can be considered when interpreting the microgenderome of zebrafish in studies investigating GI physiology and toxicology of fishes.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

707241, interní kód MU

Name: Environmental chemicals as obesogens (Acronym: OBESOGENS)

Investor: European Union, MSCA Marie Skłodowska-Curie Actions (Excellent Science)

Citovat

MARTYNIUK, Christopher J., Amanda N. BUERGER, Hana VESPALCOVÁ, Barbora RUDZANOVÁ, Shahadur R. SOHAG, Amy T. HANLON, Pamela E. GINN, Serena L. CRAFT, Soňa SMETANOVÁ, Eva BUDINSKÁ, Joseph H. Jr. BISESI and Ondřej ADAMOVSKÝ. Sex-dependent host-microbiome dynamics in zebrafish: Implications for toxicology and gastrointestinal physiology. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. NEW YORK: ELSEVIER SCIENCE INC, 2022, vol. 42, June 2022, p. 1-9. ISSN 1744-117X. Available from: https://dx.doi.org/10.1016/j.cbd.2022.100993.

@article{1883538,
   author = {Martyniuk, Christopher J. and Buerger, Amanda N. and Vespalcová, Hana and Rudzanová, Barbora and Sohag, Shahadur R. and Hanlon, Amy T. and Ginn, Pamela E. and Craft, Serena L. and Smetanová, Soňa and Budinská, Eva and Bisesi, Joseph H. Jr. and Adamovský, Ondřej},
   article_location = {NEW YORK},
   article_number = {June 2022},
   doi = {http://dx.doi.org/10.1016/j.cbd.2022.100993},
   keywords = {Sex differences; Experimental design; Gene networks; Gastrointestinal system; Microgenderome},
   language = {eng},
   issn = {1744-117X},
   journal = {Comparative Biochemistry and Physiology Part D: Genomics and Proteomics},
   title = {Sex-dependent host-microbiome dynamics in zebrafish: Implications for toxicology and gastrointestinal physiology},
   url = {https://www.sciencedirect.com/science/article/pii/S1744117X22000351?via%3Dihub},
   volume = {42},
   year = {2022}
}

TY  - JOUR
ID  - 1883538
AU  - Martyniuk, Christopher J. - Buerger, Amanda N. - Vespalcová, Hana - Rudzanová, Barbora - Sohag, Shahadur R. - Hanlon, Amy T. - Ginn, Pamela E. - Craft, Serena L. - Smetanová, Soňa - Budinská, Eva - Bisesi, Joseph H. Jr. - Adamovský, Ondřej
PY  - 2022
TI  - Sex-dependent host-microbiome dynamics in zebrafish: Implications for toxicology and gastrointestinal physiology
JF  - Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
VL  - 42
IS  - June 2022
SP  - 1-9
EP  - 1-9
PB  - ELSEVIER SCIENCE INC
SN  - 1744117X
KW  - Sex differences
KW  - Experimental design
KW  - Gene networks
KW  - Gastrointestinal system
KW  - Microgenderome
UR  - https://www.sciencedirect.com/science/article/pii/S1744117X22000351?via%3Dihub
N2  - The physiology of males and females can be vastly different, complicating interpretation of toxicological and physiological data. The objectives of this study were to elucidate the sex differences in the microbiomegastrointestinal (GI) transcriptome of adult zebrafish. We compared microbial composition and diversity in both males and females fed the same diet and housed in the same environment. There were no sex-specific differences in weight gain nor gastrointestinal morphology based on histopathology. There was no difference in gut microbial diversity, richness (Shannon and Chao1 index) nor predicted functional composition of the microbiome between males and females. Prior to post-hoc correction, male zebrafish showed higher abundance for the bacterial families Erythrobacteraceae and Lamiaceae, both belonging to the phyla Actinobacteria and Proteobacteria. At the genus level, Lamia and Altererythrobacter were more dominant in males and an unidentified genus in Bacteroidetes was more abundant in females. There were 16 unique differentially expressed transcripts in the gastrointestinal tissue between male and female zebrafish (FDR corrected, p < 0.05). Relative to males, the mRNA expression for trim35-9, slc25a48, chchd3b, csad, and hsd17b3 were lower in female GI while cyp2k6, adra2c, and bckdk were higher in the female GI. Immune and lipid-related gene network expression differed between the sexes (i.e., cholesterol export and metabolism) as well as networks related to gastric motility, gastrointestinal system absorption and digestion. Such data provide clues as to putative differences in gastrointestinal physiology between male and female zebrafish. This study identifies host-transcriptome differences that can be considered when interpreting the microgenderome of zebrafish in studies investigating GI physiology and toxicology of fishes.
ER  -

MARTYNIUK, Christopher J., Amanda N. BUERGER, Hana VESPALCOVÁ, Barbora RUDZANOVÁ, Shahadur R. SOHAG, Amy T. HANLON, Pamela E. GINN, Serena L. CRAFT, Soňa SMETANOVÁ, Eva BUDINSKÁ, Joseph H. Jr. BISESI and Ondřej ADAMOVSKÝ. Sex-dependent host-microbiome dynamics in zebrafish: Implications for toxicology and gastrointestinal physiology. \textit{Comparative Biochemistry and Physiology Part D: Genomics and Proteomics}. NEW YORK: ELSEVIER SCIENCE INC, 2022, vol.~42, June 2022, p.~1-9. ISSN~1744-117X. Available from: https://dx.doi.org/10.1016/j.cbd.2022.100993.

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