| FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO, Lenka ŠTACKOVÁ, Petr KLÁN, Hisataka KOBAYASHI and Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. Journal of the American Chemical Society. Washington: American Chemical Society, 2022, vol. 144, No 25, p. 11075-11080. ISSN 0002-7863. Available from: https://dx.doi.org/10.1021/jacs.2c02962. |
Other formats:
BibTeX
LaTeX
RIS
@article{1883610,
author = {Fukushima, Hiroshi and Matikonda, Siddharth S. and Usama, Syed Muhammad and Furusawa, Aki and Kato, Takuya and Štacková, Lenka and Klán, Petr and Kobayashi, Hisataka and Schnermann, Martin J.},
article_location = {Washington},
article_number = {25},
doi = {http://dx.doi.org/10.1021/jacs.2c02962},
keywords = {LYMPH-NODE; IN-VIVO; IDENTIFICATION; TRACKING; EGFP},
language = {eng},
issn = {0002-7863},
journal = {Journal of the American Chemical Society},
title = {Cyanine Phototruncation Enables Spatiotemporal Cell Labeling},
url = {https://pubs.acs.org/doi/10.1021/jacs.2c02962},
volume = {144},
year = {2022}
}
TY - JOUR
ID - 1883610
AU - Fukushima, Hiroshi - Matikonda, Siddharth S. - Usama, Syed Muhammad - Furusawa, Aki - Kato, Takuya - Štacková, Lenka - Klán, Petr - Kobayashi, Hisataka - Schnermann, Martin J.
PY - 2022
TI - Cyanine Phototruncation Enables Spatiotemporal Cell Labeling
JF - Journal of the American Chemical Society
VL - 144
IS - 25
SP - 11075-11080
EP - 11075-11080
PB - American Chemical Society
SN - 00027863
KW - LYMPH-NODE
KW - IN-VIVO
KW - IDENTIFICATION
KW - TRACKING
KW - EGFP
UR - https://pubs.acs.org/doi/10.1021/jacs.2c02962
N2 - Photoconvertible tracking strategies assess the dynamic migration of cell populations. Here we develop phototruncation-assisted cell tracking (PACT) and apply it to evaluate the migration of immune cells into tumor-draining lymphatics. This method is enabled by a recently discovered cyanine photoconversion reaction that leads to the two-carbon truncation and consequent blue-shift of these commonly used probes. By examining substituent effects on the heptamethine cyanine chromophore, we find that introduction of a single methoxy group increases the yield of the phototruncation reaction in neutral buffer by almost 8-fold. When converted to a membrane-bound cell-tracking variant, this probe can be applied in a series of in vitro and in vivo experiments. These include quantitative, time-dependent measurements of the migration of immune cells from tumors to tumor-draining lymph nodes. Unlike previously reported cellular photoconversion approaches, this method does not require genetic engineering and uses near-infrared (NIR) wavelengths. Overall, PACT provides a straightforward approach to label cell populations with spatiotemporal control.
ER -
FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO, Lenka ŠTACKOVÁ, Petr KLÁN, Hisataka KOBAYASHI and Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. \textit{Journal of the American Chemical Society}. Washington: American Chemical Society, 2022, vol.~144, No~25, p.~11075-11080. ISSN~0002-7863. Available from: https://dx.doi.org/10.1021/jacs.2c02962.
|
