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@article{197410,
author = {Klepárník, Karel and Malá, Zdena and Doškař, Jiří and Rosypal, Stanislav and Boček, Petr},
article_location = {Weinheim},
article_number = {4},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {An improvement of restriction analysis of bacteriophage DNA using capillary electrophoresis in agarose solution},
url = {http://www3.interscience.wiley.com/cgi-bin/abstract/110461220/ABSTRACT},
volume = {16},
year = {1995}
}
TY - JOUR
ID - 197410
AU - Klepárník, Karel - Malá, Zdena - Doškař, Jiří - Rosypal, Stanislav - Boček, Petr
PY - 1995
TI - An improvement of restriction analysis of bacteriophage DNA using capillary electrophoresis in agarose solution
JF - Electrophoresis
VL - 16
IS - 4
SP - 366
EP - 366
PB - VHC
SN - 01730835
UR - http://www3.interscience.wiley.com/cgi-bin/abstract/110461220/ABSTRACT
N2 - Phi11 and 80alpha, were examined by capillary electrophoresis (CE) for genomic homology using DNA restriction analysis. Genomic DNA of individual bacteriophages was cleaved by HindIII restriction endonuclease, and the resulting restriction fragments were separated by standard horizontal agarose slab gel electrophoresis (SGE) as well as by CE in low-melting-point agarose solutions. The number and size of restriction fragments identified by both methods were compared. The high separation power of CE makes it possible to extend the restriction fragment patterns. In most of the restriction patterns, some additional restriction fragments as small as 150 bp, not identified by SGE, were detected. With respect to speed, high separation efficiency, low sample consumption and automation, CE offers a simple procedure for processing of multiple samples cost-effectively in a reasonable time. The comparison of the complemented restriction patterns of the different phage strains and the subsequent identification of their common fragments leads to a deeper understanding of their phylogenetic relationships. The genome homologies expressed for individual phage pairs in terms of coefficient F values ranged from 15 to 69%. These values are in good accordance with the degree of DNA homology of these phages as determined by DNA hybridization studies and thermal denaturation analysis of DNA by other authors. The total size of each phage genome was estimated by adding the sizes of individual restriction fragments
ER -
KLEPÁRNÍK, Karel, Zdena MALÁ, Jiří DOŠKAŘ, Stanislav ROSYPAL a Petr BOČEK. An improvement of restriction analysis of bacteriophage DNA using capillary electrophoresis in agarose solution. Online. \textit{Electrophoresis}. Weinheim: VHC, 1995, roč.~16, č.~4, s.~366-376. ISSN~0173-0835. [citováno 2024-04-24]
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