Specific Determination of Glutathione using Micellar
Elektrokinetic Chromatography and On-Column Reaction with
2,2-dipyridyldisulphide

D 1999

Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ

Basic information

Original name

Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide

Authors

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ

Edition

Amsterdam, Netherlands, Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands, p. 10-10, 1999

Publisher

Elsevier, Netherlands

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/99:00003210

Organization unit

Faculty of Science

Keywords in English

Micellar Elektrokinetic Chromatography; On-Column Derivatization; Thiols; Glutathione

Abstract

V originále

The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.

Links

GA525/97/0340, research and development project

Name: Aktivita bakterie Thiobacilus ferrooxidans ve vztahu k ekotoxikologii sulfidických odpadů

Investor: Czech Science Foundation, Activity of Thiobacillus ferrooxidans in relation to ecotoxicology of sulfide wastes

Citovat

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide. In Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands. Amsterdam, Netherlands: Elsevier, Netherlands, 1999, p. 10.

@inproceedings{205215,
   author = {Glatz, Zdeněk and Mašlaňová, Hana},
   address = {Amsterdam, Netherlands},
   booktitle = {Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands},
   keywords = {Micellar Elektrokinetic Chromatography; On-Column Derivatization; Thiols; Glutathione},
   language = {eng},
   location = {Amsterdam, Netherlands},
   pages = {10-10},
   publisher = {Elsevier, Netherlands},
   title = {Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide},
   year = {1999}
}

TY  - JOUR
ID  - 205215
AU  - Glatz, Zdeněk - Mašlaňová, Hana
PY  - 1999
TI  - Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide
PB  - Elsevier, Netherlands
CY  - Amsterdam, Netherlands
KW  - Micellar Elektrokinetic Chromatography
KW  - On-Column Derivatization
KW  - Thiols
KW  - Glutathione
N2  - The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.
ER  -

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide. In \textit{Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands}. Amsterdam, Netherlands: Elsevier, Netherlands, 1999, p.~10.

Detailed Information on Publication Record