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@inproceedings{205215,
author = {Glatz, Zdeněk and Mašlaňová, Hana},
address = {Amsterdam, Netherlands},
booktitle = {Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands},
keywords = {Micellar Elektrokinetic Chromatography; On-Column Derivatization; Thiols; Glutathione},
language = {eng},
location = {Amsterdam, Netherlands},
pages = {10-10},
publisher = {Elsevier, Netherlands},
title = {Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide},
year = {1999}
}
TY - JOUR
ID - 205215
AU - Glatz, Zdeněk - Mašlaňová, Hana
PY - 1999
TI - Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide
PB - Elsevier, Netherlands
CY - Amsterdam, Netherlands
KW - Micellar Elektrokinetic Chromatography
KW - On-Column Derivatization
KW - Thiols
KW - Glutathione
N2 - The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.
ER -
GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide. In \textit{Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands}. Amsterdam, Netherlands: Elsevier, Netherlands. p.~10. 1999.
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