| KUDEROVÁ, Alena, Elizabeth NANAK, Martin TRUKSA and Břetislav BRZOBOHATÝ. Use of Rifampicin in T7 RNA Polymerase-Driven Expression of a Plant Enzyme. Rifampicin Improves Yield and Assembly. Protein Expression and Purification. San Diego, California: Academic Press, 1999, vol. 16, No 3, p. 405-409. ISSN 1046-5928. |
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@article{205265,
author = {Kuderová, Alena and Nanak, Elizabeth and Truksa, Martin and Brzobohatý, Břetislav},
article_location = {San Diego, California},
article_number = {3},
keywords = {rifampicin - protein expression - T7 RNA polymerase - beta-glucosidase},
language = {eng},
issn = {1046-5928},
journal = {Protein Expression and Purification},
title = {Use of Rifampicin in T7 RNA Polymerase-Driven Expression of a Plant Enzyme. Rifampicin Improves Yield and Assembly.},
volume = {16},
year = {1999}
}
TY - JOUR
ID - 205265
AU - Kuderová, Alena - Nanak, Elizabeth - Truksa, Martin - Brzobohatý, Břetislav
PY - 1999
TI - Use of Rifampicin in T7 RNA Polymerase-Driven Expression of a Plant Enzyme. Rifampicin Improves Yield and Assembly.
JF - Protein Expression and Purification
VL - 16
IS - 3
SP - 405
EP - 405
PB - Academic Press
SN - 10465928
KW - rifampicin - protein expression - T7 RNA polymerase - beta-glucosidase
N2 - Expression systems based on high selectivity and activity of T7 RNA polymerase and presence of a strong T7 promoter have been commonly used for cloning and expression of various recombinant proteins in Escherichia coli. When protein expression is not directed to periplasm, the final yield and quality of the synthesized and purified protein then depend on various factors, protein size, amino acid sequence, solubility in cytoplasm, and folding requirements among them. The yield in the T7 RNA polymerase/promoter system can be positively influenced by use of rifampicin. In this report we describe rifampicin-enhaced expression of a plant cytokinin-specific beta-glucosidase. The antibiotic not only increased the yield of the recombinant protein, which seems to be a general phenomenon, but also favored the final assembly of the protein's subunits into a catalytically active dimer form.
ER -
KUDEROVÁ, Alena, Elizabeth NANAK, Martin TRUKSA and Břetislav BRZOBOHATÝ. Use of Rifampicin in T7 RNA Polymerase-Driven Expression of a Plant Enzyme. Rifampicin Improves Yield and Assembly. \textit{Protein Expression and Purification}. San Diego, California: Academic Press, 1999, vol.~16, No~3, p.~405-409. ISSN~1046-5928.
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