| ŠMARDOVÁ, J., J. FELSBERG, Jan ŠMARDA a J. SPÍŽEK. Isolation of DNA-dependent RNA polymerase from Streptomyces granaticolor and its binding to the phage phi 29 DNA. Folia Microbiologica. Praha: Mikrobiologický ústav AV ČR, 1991, roč. 36, č. 2, s. 120-126. ISSN 0015-5632. |
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@article{209076,
author = {Šmardová, J. and Felsberg, J. and Šmarda, Jan and Spížek, J.},
article_location = {Praha},
article_number = {2},
keywords = {phi29},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Isolation of DNA-dependent RNA polymerase from Streptomyces granaticolor and its binding to the phage phi 29 DNA},
volume = {36},
year = {1991}
}
TY - JOUR
ID - 209076
AU - Šmardová, J. - Felsberg, J. - Šmarda, Jan - Spížek, J.
PY - 1991
TI - Isolation of DNA-dependent RNA polymerase from Streptomyces granaticolor and its binding to the phage phi 29 DNA
JF - Folia Microbiologica
VL - 36
IS - 2
SP - 120
EP - 120
PB - Mikrobiologický ústav AV ČR
SN - 00155632
KW - phi29
N2 - Partially purified DNA-dependent RNA polymerase of Streptomyces granaticolor was further separated on phosphocellulose in 50% glycerol and a single activity peak was obtained. The enzyme isolated in this way consisted of 4 main proteins with molar mass of 145, 132, 50 and 46kg/mol. These four subunits represented 93% proteins of the active fraction. To test the ability of RNA polymerase to recognize specific sites on DNA, binding sites for RNA polymerase on phage phi 29 DNA were mapped by electron microscopy. The specific binding sites were compared with those for RNA polymerases from Escherichia coli and Bacillus subtilis.
ER -
ŠMARDOVÁ, J., J. FELSBERG, Jan ŠMARDA a J. SPÍŽEK. Isolation of DNA-dependent RNA polymerase from Streptomyces granaticolor and its binding to the phage phi 29 DNA. \textit{Folia Microbiologica}. Praha: Mikrobiologický ústav AV ČR, 1991, roč.~36, č.~2, s.~120-126. ISSN~0015-5632.
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