Application of 2,2-Dipyridyldisulphide for Specific
Determination of Glutathione during Micellar Electrokinetic
Chromatography

D 1999

Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ

Basic information

Original name

Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography

Authors

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ

Edition

Bratislava, Proceeding of 13th International Conference, p. 25-26, 1999

Publisher

Slovenská chemická společnosť pri SAV, Bratislava

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/99:00003221

Organization unit

Faculty of Science

ISBN

1335-5236

Keywords in English

Glutathione; MEKC; 2;2-dipyridyldisulphide

Abstract

V originále

The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.

Links

GA525/97/0340, research and development project

Name: Aktivita bakterie Thiobacilus ferrooxidans ve vztahu k ekotoxikologii sulfidických odpadů

Investor: Czech Science Foundation, Activity of Thiobacillus ferrooxidans in relation to ecotoxicology of sulfide wastes

Citovat

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography. In Proceeding of 13th International Conference. Bratislava: Slovenská chemická společnosť pri SAV, Bratislava, 1999, p. 25-26. ISBN 1335-5236.

@inproceedings{213991,
   author = {Glatz, Zdeněk and Mašlaňová, Hana},
   address = {Bratislava},
   booktitle = {Proceeding of 13th International Conference},
   keywords = {Glutathione; MEKC; 2;2-dipyridyldisulphide},
   language = {eng},
   location = {Bratislava},
   isbn = {1335-5236},
   pages = {25-26},
   publisher = {Slovenská chemická společnosť pri SAV, Bratislava},
   title = {Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography},
   year = {1999}
}

TY  - JOUR
ID  - 213991
AU  - Glatz, Zdeněk - Mašlaňová, Hana
PY  - 1999
TI  - Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography
PB  - Slovenská chemická společnosť pri SAV, Bratislava
CY  - Bratislava
SN  - 13355236
KW  - Glutathione
KW  - MEKC
KW  - 2;2-dipyridyldisulphide
N2  - The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.
ER  -

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography. In \textit{Proceeding of 13th International Conference}. Bratislava: Slovenská chemická společnosť pri SAV, Bratislava, 1999, p.~25-26. ISBN~1335-5236.

Detailed Information on Publication Record