Other formats:
BibTeX
LaTeX
RIS
@article{216631, author = {Preisler, J. and Foret, F. and Karger, B. L.}, article_location = {Washington, D.C., USA}, article_number = {24}, keywords = {On-line; MALDI; TOFMS; Vacuum Deposition; Interface; Mass Spectrometry; Capillary Electrophoresis}, language = {eng}, issn = {0003-2700}, journal = {Analytical Chemistry}, title = {On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface}, volume = {70}, year = {1998} }
TY - JOUR ID - 216631 AU - Preisler, J. - Foret, F. - Karger, B. L. PY - 1998 TI - On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface JF - Analytical Chemistry VL - 70 IS - 24 SP - 5278 EP - 5278 PB - American Chemical Society SN - 00032700 KW - On-line KW - MALDI KW - TOFMS KW - Vacuum Deposition KW - Interface KW - Mass Spectrometry KW - Capillary Electrophoresis N2 - In this work, a new interface for continuous liquid on-line MALDI-TOF MS is presented. The sample, mixed with conventional matrix, is brought via a capillary inlet into the evacuated source chamber of the mass spectrometer at liquid flow rates of 200 to 400 nL/min. The liquid sample matrix is deposited on a rotating wheel, in the present study quartz, and transported to the repeller where laser desorption takes place. Rapid evaporation of the solvent (water or methanol) results in formation of a thin, ~ 50 um wide, sample trace. No crystals were observed in the trace using a scanning electron microscope, suggesting either very small sized crystals or an amorphous film. This sample uniformity resulted in excellent spot-to-spot reproducibility, with detection limits in the attomole range. Furthermore, and importantly, the interface permits the on-line coupling of high resolution microcolumn separation techniques with MALDI-MS, as demonstrated in the capillary electrophoresis - MALDI-TOF MS analysis of a 12 peptide mixture. The approach offers the potential of rapid, trace separation and analysis of complex mixtures. ER -
PREISLER, J., F. FORET and B. L. KARGER. On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 1998, vol.~70, No~24, p.~5278-5287. ISSN~0003-2700.
|