PREISLER, J., F. FORET and B. L. KARGER. On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 1998, vol. 70, No 24, p. 5278-5287. ISSN 0003-2700.
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Basic information
Original name On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface
Name in Czech On-line MALDI-TOF MS pomocí rozhraní s kontinuálním vakuovým nanášením
Authors PREISLER, J. (203 Czech Republic, guarantor), F. FORET (203 Czech Republic) and B. L. KARGER (840 United States of America).
Edition Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 1998, 0003-2700.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 4.580
Organization unit Faculty of Science
UT WoS 000077550100025
Keywords in English On-line; MALDI; TOFMS; Vacuum Deposition; Interface; Mass Spectrometry; Capillary Electrophoresis
Tags Capillary electrophoresis, interface, MALDI, mass spectrometry, On-line, TOFMS, vacuum deposition
Changed by Changed by: prof. Mgr. Jan Preisler, Ph.D., učo 45329. Changed: 28/6/2009 00:03.
Abstract
In this work, a new interface for continuous liquid on-line MALDI-TOF MS is presented. The sample, mixed with conventional matrix, is brought via a capillary inlet into the evacuated source chamber of the mass spectrometer at liquid flow rates of 200 to 400 nL/min. The liquid sample matrix is deposited on a rotating wheel, in the present study quartz, and transported to the repeller where laser desorption takes place. Rapid evaporation of the solvent (water or methanol) results in formation of a thin, ~ 50 um wide, sample trace. No crystals were observed in the trace using a scanning electron microscope, suggesting either very small sized crystals or an amorphous film. This sample uniformity resulted in excellent spot-to-spot reproducibility, with detection limits in the attomole range. Furthermore, and importantly, the interface permits the on-line coupling of high resolution microcolumn separation techniques with MALDI-MS, as demonstrated in the capillary electrophoresis - MALDI-TOF MS analysis of a 12 peptide mixture. The approach offers the potential of rapid, trace separation and analysis of complex mixtures.
Abstract (in Czech)
On-line MALDI-TOF MS pomocí rozhraní s kontinuálním vakuovým nanášením, viz anglický popis.
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