On-Line MALDI-TOF MS Using Continuous Vacuum Deposition
Interface

J 1998

On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface

PREISLER, J., F. FORET and B. L. KARGER

Basic information

Original name

On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface

Name in Czech

On-line MALDI-TOF MS pomocí rozhraní s kontinuálním vakuovým nanášením

Authors

PREISLER, J. (203 Czech Republic, guarantor), F. FORET (203 Czech Republic) and B. L. KARGER (840 United States of America)

Edition

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 1998, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.580

Organization unit

Faculty of Science

UT WoS

000077550100025

Keywords in English

On-line; MALDI; TOFMS; Vacuum Deposition; Interface; Mass Spectrometry; Capillary Electrophoresis

Abstract

ORIG CZ

V originále

In this work, a new interface for continuous liquid on-line MALDI-TOF MS is presented. The sample, mixed with conventional matrix, is brought via a capillary inlet into the evacuated source chamber of the mass spectrometer at liquid flow rates of 200 to 400 nL/min. The liquid sample matrix is deposited on a rotating wheel, in the present study quartz, and transported to the repeller where laser desorption takes place. Rapid evaporation of the solvent (water or methanol) results in formation of a thin, ~ 50 um wide, sample trace. No crystals were observed in the trace using a scanning electron microscope, suggesting either very small sized crystals or an amorphous film. This sample uniformity resulted in excellent spot-to-spot reproducibility, with detection limits in the attomole range. Furthermore, and importantly, the interface permits the on-line coupling of high resolution microcolumn separation techniques with MALDI-MS, as demonstrated in the capillary electrophoresis - MALDI-TOF MS analysis of a 12 peptide mixture. The approach offers the potential of rapid, trace separation and analysis of complex mixtures.

In Czech

On-line MALDI-TOF MS pomocí rozhraní s kontinuálním vakuovým nanášením, viz anglický popis.

Citovat

PREISLER, J., F. FORET and B. L. KARGER. On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 1998, vol. 70, No 24, p. 5278-5287. ISSN 0003-2700.

@article{216631,
   author = {Preisler, J. and Foret, F. and Karger, B. L.},
   article_location = {Washington, D.C., USA},
   article_number = {24},
   keywords = {On-line; MALDI; TOFMS; Vacuum Deposition; Interface; Mass Spectrometry; Capillary Electrophoresis},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface},
   volume = {70},
   year = {1998}
}

TY  - JOUR
ID  - 216631
AU  - Preisler, J. - Foret, F. - Karger, B. L.
PY  - 1998
TI  - On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface
JF  - Analytical Chemistry
VL  - 70
IS  - 24
SP  - 5278
EP  - 5278
PB  - American Chemical Society
SN  - 00032700
KW  - On-line
KW  - MALDI
KW  - TOFMS
KW  - Vacuum Deposition
KW  - Interface
KW  - Mass Spectrometry
KW  - Capillary Electrophoresis
N2  - In this work, a new interface for continuous liquid on-line MALDI-TOF MS is presented. The sample, mixed with conventional matrix, is brought via a capillary inlet into the evacuated source chamber of the mass spectrometer at liquid flow rates of 200 to 400 nL/min. The liquid sample matrix is deposited on a rotating wheel, in the present study quartz, and transported to the repeller where laser desorption takes place. Rapid evaporation of the solvent (water or methanol) results in formation of a thin, ~ 50 um wide, sample trace. No crystals were observed in the trace using a scanning electron microscope, suggesting either very small sized crystals or an amorphous film. This sample uniformity resulted in excellent spot-to-spot reproducibility, with detection limits in the attomole range. Furthermore, and importantly, the interface permits the on-line coupling of high resolution microcolumn separation techniques with MALDI-MS, as demonstrated in the capillary electrophoresis - MALDI-TOF MS analysis of a 12 peptide mixture. The approach offers the potential of rapid, trace separation and analysis of complex mixtures.
ER  -

PREISLER, J., F. FORET and B. L. KARGER. On-Line MALDI-TOF MS Using Continuous Vacuum Deposition Interface. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 1998, vol.~70, No~24, p.~5278-5287. ISSN~0003-2700.

Detailed Information on Publication Record