Laser Photodissociation of Insulin Ions Generated by Matrix-Assisted Laser Desorption

PREISLER, J. and E. S. YEUNG. Laser Photodissociation of Insulin Ions Generated by Matrix-Assisted Laser Desorption. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 1997, vol. 69, No 21, p. 4390-4398. ISSN 0003-2700.

Basic information

Original name

Laser Photodissociation of Insulin Ions Generated by Matrix-Assisted Laser Desorption

Name in Czech

Laserová fotodisociace insulinových iontů tvořených laserovou desorpcí za účasti matrice

Authors

PREISLER, J. (203 Czech Republic, guarantor) and E. S. YEUNG (840 United States of America)

Edition

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 1997, 0003-2700

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.743

Organization unit

Faculty of Science

UT WoS

A1997YD67800020

Keywords in English

laser; photodissociation; MALDI; matrix; desorption; insulin;

Tags

Desorption, insulin, laser, MALDI, matrix, photodissociation

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Abstract

V originále

A novel method for studying the ionization step of matrixassisted laser desorption/ionization (MALDI) is demonstrated. A 193-nm pulse from an ArF excimer laser is used to photodissociate a portion of a plume of insulin ions generated by MALDI. Laser photodissociation (LPD) creates a "hole", i.e., a negative spike in the insulin peak in the time-of-flight (TOF) mass spectrum. The position of the hole in the mass spectrum provides useful measurements of the characteristics (position, time, and velocity) of insulin ions shortly after their creation. Although the performance of the method can be further improved, the data obtained could be used to refine our current understanding of MALDI and to improve the resolution of MALDI-TOFMS.