Characterization of Non-Bonded Poly(Ethylene Oxide) Coating for
Capillary Electrophoresis via Continuous Monitoring of
Electroosmotic Flow

J 1996

Characterization of Non-Bonded Poly(Ethylene Oxide) Coating for Capillary Electrophoresis via Continuous Monitoring of Electroosmotic Flow

PREISLER, J. and E. S. YEUNG

Basic information

Original name

Characterization of Non-Bonded Poly(Ethylene Oxide) Coating for Capillary Electrophoresis via Continuous Monitoring of Electroosmotic Flow

Authors

PREISLER, J. (203 Czech Republic, guarantor) and E. S. YEUNG (840 United States of America)

Edition

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 1996, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

UT WoS

A1996VE59800032

Keywords in English

polyethyleneoxid; poly(ethyleneoxid); electroosmotic; capillary electrophoresis; CCD; coating

Abstract

V originále

We examined changes in a poly(ethylene oxide) (PEO) coating by continuously monitoring the electroosmotic flow (EOF) in a fused-silica capillary during electrophoresis. An imaging CCD camera was used to follow the motion of a fluorescent neutral marker zone along the length of the capillary. The PEO coating was shown to reduce the velocity of EOF by more than 1 order of magnitude compared to a bare capillary at pH 7.0. However, it did not reduce EOF efficiently at pH 8.2. The coating protocol was important, especially at an intermediate pH of 7.7. Capillary reconditioning with an acidified solution of PEO was necessary in order to create a stable and efficient coating. In all cases we observed a gradual increase of EOF during extended runs, suggesting that the coating is slowly being degraded. The increase of pH in the cathodic (detection-end) buffer reservoir beyond pH ~8.0, e.g., as a result of electrolysis, had a large impact on the stability of the coating. This phenomenon may be used for the efficient and fast regeneration of the column surface and provides a simpler and more reliable alternative to pressure flushing of the capillary.

Citovat

PREISLER, J. and E. S. YEUNG. Characterization of Non-Bonded Poly(Ethylene Oxide) Coating for Capillary Electrophoresis via Continuous Monitoring of Electroosmotic Flow (Laser Photodissociation of Insulin Ions Generated by Matrix-Assisted Laser Desorption). Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 1996, vol. 68, No 17, p. 2885-2889. ISSN 0003-2700.

@article{216672,
   author = {Preisler, J. and Yeung, E. S.},
   article_location = {Washington, D.C., USA},
   article_number = {17},
   keywords = {polyethyleneoxid; poly(ethyleneoxid); electroosmotic; capillary electrophoresis; CCD; coating},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Characterization of Non-Bonded Poly(Ethylene Oxide) Coating for Capillary Electrophoresis via Continuous Monitoring of Electroosmotic Flow},
   volume = {68},
   year = {1996}
}

TY  - JOUR
ID  - 216672
AU  - Preisler, J. - Yeung, E. S.
PY  - 1996
TI  - Characterization of Non-Bonded Poly(Ethylene Oxide) Coating for Capillary Electrophoresis via Continuous Monitoring of Electroosmotic Flow
JF  - Analytical Chemistry
VL  - 68
IS  - 17
SP  - 2885
EP  - 2885
PB  - American Chemical Society
SN  - 00032700
KW  - polyethyleneoxid
KW  - poly(ethyleneoxid)
KW  - electroosmotic
KW  - capillary electrophoresis
KW  - CCD
KW  - coating
N2  - We examined changes in a poly(ethylene oxide) (PEO) coating by continuously monitoring the electroosmotic flow (EOF) in a fused-silica capillary during electrophoresis. An imaging CCD camera was used to follow the motion of a fluorescent neutral marker zone along the length of the capillary. The PEO coating was shown to reduce the velocity of EOF by more than 1 order of magnitude compared to a bare capillary at pH 7.0. However, it did not reduce EOF efficiently at pH 8.2. The coating protocol was important, especially at an intermediate pH of 7.7. Capillary reconditioning with an acidified solution of PEO was necessary in order to create a stable and efficient coating. In all cases we observed a gradual increase of EOF during extended runs, suggesting that the coating is slowly being degraded. The increase of pH in the cathodic (detection-end) buffer reservoir beyond pH ~8.0, e.g., as a result of electrolysis, had a large impact on the stability of the coating. This phenomenon may be used for the efficient and fast regeneration of the column surface and provides a simpler and more reliable alternative to pressure flushing of the capillary.
ER  -

PREISLER, J. and E. S. YEUNG. Characterization of Non-Bonded Poly(Ethylene Oxide) Coating for Capillary Electrophoresis via Continuous Monitoring of Electroosmotic Flow (Laser Photodissociation of Insulin Ions Generated by Matrix-Assisted Laser Desorption). \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 1996, vol.~68, No~17, p.~2885-2889. ISSN~0003-2700.

Detailed Information on Publication Record