PREISLER, J. and E. S. YEUNG. Characterization of Matrix-Assisted Laser Desorption Based on Absorption and Acoustic Monitoring. Applied Spectroscopy. USA: Society Appl. Spectroscopy, 1995, vol. 49, No 12, p. 1826-1833. ISSN 0003-7028.
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Basic information
Original name Characterization of Matrix-Assisted Laser Desorption Based on Absorption and Acoustic Monitoring
Authors PREISLER, J. (203 Czech Republic, guarantor) and E. S. YEUNG (840 United States of America).
Edition Applied Spectroscopy, USA, Society Appl. Spectroscopy, 1995, 0003-7028.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Organization unit Faculty of Science
UT WoS A1995TM58600021
Keywords in English MALDI; desorption; laser; acoustic; photoacoustic; deflect
Tags acoustic, deflect, Desorption, laser, MALDI, photoacoustic
Changed by Changed by: prof. Mgr. Jan Preisler, Ph.D., učo 45329. Changed: 28/6/2009 00:05.
Abstract
Conventional methods for studying matrix-assisted desorption/ionization rely on mass spectroscopy. In this research, a 488-nm argon-ion laser beam is deflected by two acoustooptic deflectors to image plumes desorbed at atmospheric pressure via absorption. All species, including neutral molecules, are monitored. Interesting features, e.g. differences between the initial plume and subsequent plumes desorbed from the same spot, or the formation of two plumes from one laser shot are observed. Total plume absorbance can be correlated with the acoustic signal generated by the desorption event. A model equation for the plume velocity as a function of time is proposed. Optical probing also enables accurate determination of plume velocities at reduced pressures. These results define the optimal conditions for desorbing analytes from matrices, as opposed to achieving a compromise between efficient desorption and efficient ionization as is practiced in mass spectrometry.
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