Sequencing Independent Molecular Typing of Staphylococcus
aureus Isolates: Approach for Infection Control and Clonal
Characterization

J 2022

Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization

DUFKOVÁ, Kristýna, Matěj BEZDÍČEK, Kristina CUPROVA, Dagmar PANTUCKOVA, Marketa NYKRYNOVA et. al.

Basic information

Original name

Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization

Authors

DUFKOVÁ, Kristýna (203 Czech Republic, belonging to the institution), Matěj BEZDÍČEK (203 Czech Republic, belonging to the institution), Kristina CUPROVA (203 Czech Republic), Dagmar PANTUCKOVA (203 Czech Republic), Marketa NYKRYNOVA (203 Czech Republic), Eva BRHELOVA (203 Czech Republic), Iva KOCMANOVA (203 Czech Republic), Silvie HODOVA (203 Czech Republic), Marketa HANSLIANOVA (203 Czech Republic), Tomas JUREN (203 Czech Republic), Břetislav LIPOVÝ (203 Czech Republic), Jiří MAYER (203 Czech Republic, belonging to the institution) and Martina LENGEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Microbiology Spectrum, WASHINGTON, American Society for Microbiology, 2022, 2165-0497

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.700

RIV identification code

RIV/00216224:14110/22:00126414

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1128/spectrum.01817-21

UT WoS

000766015800131

Keywords in English

MLST; MRSA; MSSA; high-resolution melting; mini-MLST; spa-typing; whole-genome sequencing

Abstract

V originále

Staphylococcus aureus is a major bacterial human pathogen that causes a wide variety of clinical manifestations. The main aim of the presented study was to determine and optimize a novel sequencing independent approach that enables molecular typing of S. aureus isolates and elucidates the transmission of emergent clones between patients. In total, 987 S. aureus isolates including both methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates were used to evaluate the novel typing approach combining high-resolution melting (HRM) analysis of multilocus sequence typing (MLST) genes (mini-MLST) and spa gene (spa-HRM). The novel approach's discriminatory ability was evaluated by whole-genome sequencing (WGS). The clonal relatedness of tested isolates was set by the BURP and BURST approach using spa and MLST data, respectively. Mini-MLST classified the S. aureus isolates into 38 clusters, followed by spa-HRM classifying the isolates into 101 clusters. The WGS proved HRM-based methods to effectively differentiate between related S. aureus isolates. Visualizing evolutionary relationships among different spa-types provided by the BURP algorithm showed comparable results to MLST/mini-MLST clonal clusters. We proved that the combination of mini-MLST and spa-HRM is rapid, reproducible, and cost-efficient. In addition to high discriminatory ability, the correlation between spa evolutionary relationships and mini-MLST clustering allows the variability in population structure to be monitored. IMPORTANCE Rapid and cost-effective molecular typing tools for Staphylococcus aureus epidemiological applications such as transmission tracking, source attribution and outbreak investigations are highly desirable. High-resolution melting based methods are effective alternative to those based on sequencing. Their good reproducibility and easy performance allow prospective typing of large set of isolates while reaching great discriminatory power. In this study, we established a new epidemiological approach to S. aureus typing. This scheme has the potential to greatly improve epidemiological investigations of S. aureus.

Links

EF19_073/0016943, research and development project

Name: Interní grantová agentura Masarykovy univerzity

MUNI/A/1330/2021, interní kód MU

Name: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit IX (Acronym: VýDiTeHeMa IX)

Investor: Masaryk University

MUNI/IGA/1345/2020, interní kód MU

Name: The Development and Application of Fast MLST-based Typing Method for Multi-drug Resistant Bacteria Outbreak Detection

Investor: Masaryk University

Citovat

DUFKOVÁ, Kristýna, Matěj BEZDÍČEK, Kristina CUPROVA, Dagmar PANTUCKOVA, Marketa NYKRYNOVA, Eva BRHELOVA, Iva KOCMANOVA, Silvie HODOVA, Marketa HANSLIANOVA, Tomas JUREN, Břetislav LIPOVÝ, Jiří MAYER and Martina LENGEROVÁ. Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization. Microbiology Spectrum. WASHINGTON: American Society for Microbiology, 2022, vol. 10, No 1, p. 1-10. ISSN 2165-0497. Available from: https://dx.doi.org/10.1128/spectrum.01817-21.

@article{2211021,
   author = {Dufková, Kristýna and Bezdíček, Matěj and Cuprova, Kristina and Pantuckova, Dagmar and Nykrynova, Marketa and Brhelova, Eva and Kocmanova, Iva and Hodova, Silvie and Hanslianova, Marketa and Juren, Tomas and Lipový, Břetislav and Mayer, Jiří and Lengerová, Martina},
   article_location = {WASHINGTON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1128/spectrum.01817-21},
   keywords = {MLST; MRSA; MSSA; high-resolution melting; mini-MLST; spa-typing; whole-genome sequencing},
   language = {eng},
   issn = {2165-0497},
   journal = {Microbiology Spectrum},
   title = {Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization},
   url = {https://journals.asm.org/doi/10.1128/spectrum.01817-21},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 2211021
AU  - Dufková, Kristýna - Bezdíček, Matěj - Cuprova, Kristina - Pantuckova, Dagmar - Nykrynova, Marketa - Brhelova, Eva - Kocmanova, Iva - Hodova, Silvie - Hanslianova, Marketa - Juren, Tomas - Lipový, Břetislav - Mayer, Jiří - Lengerová, Martina
PY  - 2022
TI  - Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization
JF  - Microbiology Spectrum
VL  - 10
IS  - 1
SP  - 1-10
EP  - 1-10
PB  - American Society for Microbiology
SN  - 21650497
KW  - MLST
KW  - MRSA
KW  - MSSA
KW  - high-resolution melting
KW  - mini-MLST
KW  - spa-typing
KW  - whole-genome sequencing
UR  - https://journals.asm.org/doi/10.1128/spectrum.01817-21
N2  - Staphylococcus aureus is a major bacterial human pathogen that causes a wide variety of clinical manifestations. The main aim of the presented study was to determine and optimize a novel sequencing independent approach that enables molecular typing of S. aureus isolates and elucidates the transmission of emergent clones between patients. In total, 987 S. aureus isolates including both methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates were used to evaluate the novel typing approach combining high-resolution melting (HRM) analysis of multilocus sequence typing (MLST) genes (mini-MLST) and spa gene (spa-HRM). The novel approach's discriminatory ability was evaluated by whole-genome sequencing (WGS). The clonal relatedness of tested isolates was set by the BURP and BURST approach using spa and MLST data, respectively. Mini-MLST classified the S. aureus isolates into 38 clusters, followed by spa-HRM classifying the isolates into 101 clusters. The WGS proved HRM-based methods to effectively differentiate between related S. aureus isolates. Visualizing evolutionary relationships among different spa-types provided by the BURP algorithm showed comparable results to MLST/mini-MLST clonal clusters. We proved that the combination of mini-MLST and spa-HRM is rapid, reproducible, and cost-efficient. In addition to high discriminatory ability, the correlation between spa evolutionary relationships and mini-MLST clustering allows the variability in population structure to be monitored. IMPORTANCE Rapid and cost-effective molecular typing tools for Staphylococcus aureus epidemiological applications such as transmission tracking, source attribution and outbreak investigations are highly desirable. High-resolution melting based methods are effective alternative to those based on sequencing. Their good reproducibility and easy performance allow prospective typing of large set of isolates while reaching great discriminatory power. In this study, we established a new epidemiological approach to S. aureus typing. This scheme has the potential to greatly improve epidemiological investigations of S. aureus.
ER  -

DUFKOVÁ, Kristýna, Matěj BEZDÍČEK, Kristina CUPROVA, Dagmar PANTUCKOVA, Marketa NYKRYNOVA, Eva BRHELOVA, Iva KOCMANOVA, Silvie HODOVA, Marketa HANSLIANOVA, Tomas JUREN, Břetislav LIPOVÝ, Jiří MAYER and Martina LENGEROVÁ. Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization. \textit{Microbiology Spectrum}. WASHINGTON: American Society for Microbiology, 2022, vol.~10, No~1, p.~1-10. ISSN~2165-0497. Available from: https://dx.doi.org/10.1128/spectrum.01817-21.

Detailed Information on Publication Record