Detailed Information on Publication Record
2022
Pharmacokinetic profiling via dried blood spot sampling method
PELCOVÁ, Marta, Eva KREJČÍŘOVÁ, Jan JUŘICA and Zdeněk GLATZBasic information
Original name
Pharmacokinetic profiling via dried blood spot sampling method
Name in Czech
Farmakokinetické profilování pomocí metody suché krevní kapky
Name (in English)
Pharmacokinetic profiling via dried blood spot sampling method
Authors
Edition
Advances in Chromatography and Electrophoresis & Chiranal 2022, 2022
Other information
Type of outcome
Konferenční abstrakt
Confidentiality degree
není předmětem státního či obchodního tajemství
Keywords (in Czech)
farmakokinetika, klozapin, suchá krevní kapka, preklinická studie
Keywords in English
pharmacokinetics, clozapine, dried blood spot, preclinical study
Změněno: 22/8/2022 11:00, Mgr. Marta Pelcová, Ph.D.
V originále
Pharmacokinetic profiles are still an inevitable part of preclinical safety studies. Nowadays, animal models are subjected to ethical standards to be carefully monitored and the principles of the 3Rs are required: replacement, reduction and refinement. Use of dried blood spot (DBS) samples, typically 20-50 µl, rather than the larger blood volumes required to obtain plasma samples, fully embraces the latter two principles of reduction and refinement. We optimized and validated RP-HPLC method (Kinetex C18 column, 100x3.0 mm, 2.6 μm, Phenomenex) hyphenated to mass spectrometry (maXis impact qTOF, Bruker Daltonics) for the determination of two antipsychotics (namely olanzapine and clozapine) and an antidepressant (mirtazapine) in rat DBS samples. The developed LC-MS method offers short analysis cycle time (13 min) with satisfactory sensitivity covering concentration range expected for a pharmacokinetic profile at chosen drug dose. DBS samples for all time points were acquired from just one body, quantified and resultant profiles were figured.
In Czech
Famakokinetický profil léčiva je stále nedílnou součástí preklinických studií. V současnosti preklinické studie na zvířatech jsou podrobně sledovány a podléhají principům 3R, tj. náhrada jinou metodou, snížení počtu zvířat a zjemnění bolesti. Užití metody suché krevní kapky s typickým odběrem 20-50 ul krve je ve shodě s principy snížení a zjemnění bolesti. Optimalizovali a validovali jsme RP-HPLC metodu s hmotnostně spektrometrickou detekcí pro stanovení dvou antipsychotik (olanzapin a klozapin) a antidepresiva ( mirtazapin) v suché krevní kapce krysí krve. Vzvinutá LC-MS metoda nabíyí krátký čas analýzy s dostatečnou citlivostí pokrývající koncentrační rozsah očekávaný při zvolené dávce zvoleného léčiva (klozapin). Všechny DBS vzorky byly odebrány z jednoho jediného těla pokusného zvířete, kvantifikovány a byl získán výsledný profil a odpovídající fakmakokinetické parametry.
In English
Pharmacokinetic profiles are still an inevitable part of preclinical safety studies. Nowadays, animal models are subjected to ethical standards to be carefully monitored and the principles of the 3Rs are required: replacement, reduction and refinement. Use of dried blood spot (DBS) samples, typically 20-50 µl, rather than the larger blood volumes required to obtain plasma samples, fully embraces the latter two principles of reduction and refinement. We optimized and validated RP-HPLC method (Kinetex C18 column, 100x3.0 mm, 2.6 μm, Phenomenex) hyphenated to mass spectrometry (maXis impact qTOF, Bruker Daltonics) for the determination of two antipsychotics (namely olanzapine and clozapine) and an antidepressant (mirtazapine) in rat DBS samples. The developed LC-MS method offers short analysis cycle time (13 min) with satisfactory sensitivity covering concentration range expected for a pharmacokinetic profile at chosen drug dose. DBS samples for all time points were acquired from just one body, quantified and resultant profiles were figured.
Links
| MUNI/G/1464/2018, interní kód MU |
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