J 2022

The Absence of Retroelement Activity Is Characteristic for Childhood Acute Leukemias and Adult Acute Lymphoblastic Leukemia

URAZBAKHTIN, Shamil, Anastasia SMIRNOVA, Anastasiya VOLAKHAVA, Elena ZERKALENKOVA, Maria SALYUTINA et. al.

Basic information

Original name

The Absence of Retroelement Activity Is Characteristic for Childhood Acute Leukemias and Adult Acute Lymphoblastic Leukemia

Authors

URAZBAKHTIN, Shamil, Anastasia SMIRNOVA, Anastasiya VOLAKHAVA (112 Belarus, belonging to the institution), Elena ZERKALENKOVA, Maria SALYUTINA, Michael DOUBEK (203 Czech Republic, belonging to the institution), Hana JELINKOVA, Nelly KHUDAINAZAROVA, Egor VOLCHKOV, Laima BELYAEVA, Ekaterina KOMECH, Šárka PAVLOVÁ (203 Czech Republic, belonging to the institution), Yuri LEBEDEV, Karla PLEVOVÁ (203 Czech Republic, belonging to the institution), Yulia OLSHANSKAYA, Alexander KOMKOV and Ilgar MAMEDOV (643 Russian Federation, belonging to the institution)

Edition

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2022, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.600

RIV identification code

RIV/00216224:14740/22:00129135

Organization unit

Central European Institute of Technology

UT WoS

000755014800001

Keywords in English

acute leukemia; retroelements; mobile elements; tumor-specific insertions

Tags

International impact, Reviewed
Změněno: 3/4/2023 10:01, Mgr. Pavla Foltynová, Ph.D.

Abstract

V originále

Retroelements (RE) have been proposed as important players in cancerogenesis. Different cancer types are characterized by a different level of tumor-specific RE insertions. In previous studies, small cohorts of hematological malignancies, such as acute myeloid leukemia, multiple myeloma, and chronic lymphocytic leukemia have been characterized by a low level of RE insertional activity. Acute lymphoblastic leukemia (ALL) in adults and childhood acute leukemias have not been studied in this context. We performed a search for new RE insertions (Alu and L1) in 44 childhood ALL, 14 childhood acute myeloid leukemia, and 14 adult ALL samples using a highly sensitive NGS-based approach. First, we evaluated the method sensitivity revealing the 1% detection threshold for the proportion of cells with specific RE insertion. Following this result, we did not identify new tumor-specific RE insertions in the tested cohort of acute leukemia samples at the established level of sensitivity. Additionally, we analyzed the transcription levels of active L1 copies and found them increased. Thus, the increased transcription of active L1 copies is not sufficient for overt elevation of L1 retrotranspositional activity in leukemia.

Links

GA19-11299S, research and development project
Name: Úloha transpozibilních elementů u hematologických malignit
Investor: Czech Science Foundation