Detailed Information on Publication Record
2022
The Absence of Retroelement Activity Is Characteristic for Childhood Acute Leukemias and Adult Acute Lymphoblastic Leukemia
URAZBAKHTIN, Shamil, Anastasia SMIRNOVA, Anastasiya VOLAKHAVA, Elena ZERKALENKOVA, Maria SALYUTINA et. al.Basic information
Original name
The Absence of Retroelement Activity Is Characteristic for Childhood Acute Leukemias and Adult Acute Lymphoblastic Leukemia
Authors
URAZBAKHTIN, Shamil, Anastasia SMIRNOVA, Anastasiya VOLAKHAVA (112 Belarus, belonging to the institution), Elena ZERKALENKOVA, Maria SALYUTINA, Michael DOUBEK (203 Czech Republic, belonging to the institution), Hana JELINKOVA, Nelly KHUDAINAZAROVA, Egor VOLCHKOV, Laima BELYAEVA, Ekaterina KOMECH, Šárka PAVLOVÁ (203 Czech Republic, belonging to the institution), Yuri LEBEDEV, Karla PLEVOVÁ (203 Czech Republic, belonging to the institution), Yulia OLSHANSKAYA, Alexander KOMKOV and Ilgar MAMEDOV (643 Russian Federation, belonging to the institution)
Edition
International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2022, 1422-0067
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.600
RIV identification code
RIV/00216224:14740/22:00129135
Organization unit
Central European Institute of Technology
UT WoS
000755014800001
Keywords in English
acute leukemia; retroelements; mobile elements; tumor-specific insertions
Tags
International impact, Reviewed
Změněno: 3/4/2023 10:01, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Retroelements (RE) have been proposed as important players in cancerogenesis. Different cancer types are characterized by a different level of tumor-specific RE insertions. In previous studies, small cohorts of hematological malignancies, such as acute myeloid leukemia, multiple myeloma, and chronic lymphocytic leukemia have been characterized by a low level of RE insertional activity. Acute lymphoblastic leukemia (ALL) in adults and childhood acute leukemias have not been studied in this context. We performed a search for new RE insertions (Alu and L1) in 44 childhood ALL, 14 childhood acute myeloid leukemia, and 14 adult ALL samples using a highly sensitive NGS-based approach. First, we evaluated the method sensitivity revealing the 1% detection threshold for the proportion of cells with specific RE insertion. Following this result, we did not identify new tumor-specific RE insertions in the tested cohort of acute leukemia samples at the established level of sensitivity. Additionally, we analyzed the transcription levels of active L1 copies and found them increased. Thus, the increased transcription of active L1 copies is not sufficient for overt elevation of L1 retrotranspositional activity in leukemia.
Links
GA19-11299S, research and development project |
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