Effect of Polymeric Nanoparticles with Entrapped Fish Oil or
Mupirocin on Skin Wound Healing Using a Porcine Model

J 2022

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

KOMPRDA, Tomas, Zbysek SLADEK, Monika VICENOVA, Jana SIMONOVA, Gabriela FRANKE et. al.

Basic information

Original name

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

Authors

KOMPRDA, Tomas (203 Czech Republic, guarantor), Zbysek SLADEK (203 Czech Republic), Monika VICENOVA (203 Czech Republic), Jana SIMONOVA (203 Czech Republic), Gabriela FRANKE (203 Czech Republic), Katarina KACVINSKA (203 Czech Republic), Cristina SABLIOV, Carlos E ASTETE, Lenka LEVA (203 Czech Republic), Vendula POPELKOVA (203 Czech Republic), Andrej BATIK (203 Czech Republic), Lucy VOJTOVA (203 Czech Republic), Břetislav LIPOVÝ (203 Czech Republic, belonging to the institution) and Milena MATEJOVICOVA (203 Czech Republic)

Edition

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2022, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30212 Surgery

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.600

RIV identification code

RIV/00216224:14110/22:00126528

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijms23147663

UT WoS

000832282300001

Keywords in English

cutaneous wounds; polyunsaturated fatty acids n-3; nanoparticles; poly(lactic-co-glycolic) acid; mupirocin; collagen; hydroxyproline; cyclooxygenase-2; transforming growth factor

Abstract

V originále

The utilization of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) loaded in collagen-based scaffolds for cutaneous wound healing using a porcine model is unique for the present study. Full-depth cutaneous excisions (5 x 5 cm) on the pig dorsa were treated with pure collagen scaffold (control, C), empty PLGA NPs (NP), FO, mupirocin (MUP), PLGA NPs with entrapped FO (NP/FO) and PLGA NPs with entrapped MUP (NP/MUP). The following markers were evaluated on days 0, 3, 7, 14 and 21 post-excision: collagen, hydroxyproline (HP), angiogenesis and expressions of the COX2, EGF, COL1A1, COL1A3, TGFB1, VEGFA, CCL5 and CCR5 genes. The hypothesis that NP/FO treatment is superior to FO alone and that it is comparable to NP/MUP was tested. NP/FO treatment increased HP in comparison with both FO alone and NP/MUP (day 14) but decreased (p < 0.05) angiogenesis in comparison with FO alone (day 3). NP/FO increased (p < 0.05) the expression of the CCR5 gene (day 3) and tended (p > 0.05) to increase the expressions of the EGF (day 7, day 14), TGFB1 (day 21) and CCL5 (day 7, day 21) genes as compared with NP/MUP. NP/FO can be suggested as a suitable alternative to NP/MUP in cutaneous wound treatment.

Citovat

KOMPRDA, Tomas, Zbysek SLADEK, Monika VICENOVA, Jana SIMONOVA, Gabriela FRANKE, Katarina KACVINSKA, Cristina SABLIOV, Carlos E ASTETE, Lenka LEVA, Vendula POPELKOVA, Andrej BATIK, Lucy VOJTOVA, Břetislav LIPOVÝ and Milena MATEJOVICOVA. Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2022, vol. 23, No 14, p. 1-20. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms23147663.

@article{2213738,
   author = {Komprda, Tomas and Sladek, Zbysek and Vicenova, Monika and Simonova, Jana and Franke, Gabriela and Kacvinska, Katarina and Sabliov, Cristina and Astete, Carlos E and Leva, Lenka and Popelkova, Vendula and Batik, Andrej and Vojtova, Lucy and Lipový, Břetislav and Matejovicova, Milena},
   article_location = {Basel},
   article_number = {14},
   doi = {http://dx.doi.org/10.3390/ijms23147663},
   keywords = {cutaneous wounds; polyunsaturated fatty acids n-3; nanoparticles; poly(lactic-co-glycolic) acid; mupirocin; collagen; hydroxyproline; cyclooxygenase-2; transforming growth factor},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model},
   url = {https://www.mdpi.com/1422-0067/23/14/7663},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 2213738
AU  - Komprda, Tomas - Sladek, Zbysek - Vicenova, Monika - Simonova, Jana - Franke, Gabriela - Kacvinska, Katarina - Sabliov, Cristina - Astete, Carlos E - Leva, Lenka - Popelkova, Vendula - Batik, Andrej - Vojtova, Lucy - Lipový, Břetislav - Matejovicova, Milena
PY  - 2022
TI  - Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model
JF  - International Journal of Molecular Sciences
VL  - 23
IS  - 14
SP  - 1-20
EP  - 1-20
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - cutaneous wounds
KW  - polyunsaturated fatty acids n-3
KW  - nanoparticles
KW  - poly(lactic-co-glycolic) acid
KW  - mupirocin
KW  - collagen
KW  - hydroxyproline
KW  - cyclooxygenase-2
KW  - transforming growth factor
UR  - https://www.mdpi.com/1422-0067/23/14/7663
N2  - The utilization of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) loaded in collagen-based scaffolds for cutaneous wound healing using a porcine model is unique for the present study. Full-depth cutaneous excisions (5 x 5 cm) on the pig dorsa were treated with pure collagen scaffold (control, C), empty PLGA NPs (NP), FO, mupirocin (MUP), PLGA NPs with entrapped FO (NP/FO) and PLGA NPs with entrapped MUP (NP/MUP). The following markers were evaluated on days 0, 3, 7, 14 and 21 post-excision: collagen, hydroxyproline (HP), angiogenesis and expressions of the COX2, EGF, COL1A1, COL1A3, TGFB1, VEGFA, CCL5 and CCR5 genes. The hypothesis that NP/FO treatment is superior to FO alone and that it is comparable to NP/MUP was tested. NP/FO treatment increased HP in comparison with both FO alone and NP/MUP (day 14) but decreased (p < 0.05) angiogenesis in comparison with FO alone (day 3). NP/FO increased (p < 0.05) the expression of the CCR5 gene (day 3) and tended (p > 0.05) to increase the expressions of the EGF (day 7, day 14), TGFB1 (day 21) and CCL5 (day 7, day 21) genes as compared with NP/MUP. NP/FO can be suggested as a suitable alternative to NP/MUP in cutaneous wound treatment.
ER  -

KOMPRDA, Tomas, Zbysek SLADEK, Monika VICENOVA, Jana SIMONOVA, Gabriela FRANKE, Katarina KACVINSKA, Cristina SABLIOV, Carlos E ASTETE, Lenka LEVA, Vendula POPELKOVA, Andrej BATIK, Lucy VOJTOVA, Břetislav LIPOVÝ and Milena MATEJOVICOVA. Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2022, vol.~23, No~14, p.~1-20. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms23147663.

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